Oxidative iodination of human lactoferrin (Lf) as commonly performed by using the chloramine-T, the Iodogen or the lactoperoxidase method produces an unreliable tracer protein because of excessive and heterogeneous polymer formation. Before iodination a minor tetramer fraction may be demonstrable in iron-saturated Lf only. Iodination-induced polymerization of iron-poor as well as iron-saturated...

The time required to visualize proteins using Coomassie Blue dye has been significantly reduced with the introduction of fast staining protocols based on staining with a Coomassie Blue dye solution at boiling temperatures. However, fast stainings suffer from high gel backgrounds, reducing the signal-to-noise ratio and limiting the number of detectable spots in the case of 2D SDS-PAGE. The aim o...

Abstracts The addition of polyphenols to ice cream mix has been shown cause a decrease in melting rate that cream, although the mechanisms this effect are not well understood. To better understand phenomenon, objective study was effects polyphenol, tannic acid (TA), on dairy cream. TA added at 0.75%, 1.5% and 3% (wt/wt) concentrations. An increase complex viscosity seen as TA% increased. pH pro...

A novel gene coding for an endo-beta-1,4-mannanase (manA) from Bacillus subtilis strain G1 was cloned and overexpressed in P. pastoris GS115, and the enzyme was purified and characterized. The manA gene consisted of an open reading frame of 1,092 nucleotides, encoding a 364-aa protein, with a predicted molecular mass of 41 kDa. The beta-mannanase showed an identity of 90.2-92.9% (< or =95%) wit...

The effect of the polycation polyethyleneimine (PEI) on the permeability properties of the Gram-negative bacterial outer membrane was investigated using Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium as target organisms. At concentrations of less than 20 micrograms ml-1, PEI increased the bacterial uptake of 1-N-phenylnaphthylamine, which is a hydrophobic probe whose quantu...

ECM protein enrichment and immunoblotting 100mg of frozen samples were homogenized using a polytron (Kinematica, Bohemia, NY) in 250μL of buffer C (HEPES pH7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium OrthoVanadate) of the CNMCS protein extraction kit. Lysates were then spun for 20 min. at 15,000 rpm at 4°C, the supernatant was saved (intermediate fraction 1, enriched for cytosolic proteins...

Considerable interest has been focused on the role of myosin light chain LC(2) in the contraction of vertebrate striated muscle. A study was undertaken to further our investigations (Moss, R.L., G.G. Giulian, and M.L. Greaser, 1981, J. Biol. Chem., 257:8588-8591) of the effects of LC(2) removal upon contraction in skinned fibers from rabbit psoas muscles. Isometric tension and maximum velocity ...

Cereal Chem. 70(1):22-26 A direct colorimetric method that simultaneously combines measurematerial, absorbance at 412 nm was read. This assay was highly reproment of solubilized and insoluble thiol groups and disulfide bonds in ducible, and measurements agreed with direct amino acid analysis. Twincorn meal-based materials is described. Ellman's reagent, 5,5'-dithiobis screw extrusion of corn me...