Homologous recombination was investigated in Escherichia coli with two plasmids, each carrying the homologous region (two defective neo genes, one with an amino-end deletion and the other with a carboxyl-end deletion) in either direct or inverted orientation. Recombination efficiency was measured in recBC sbcBC and recBC sbcA strains in three ways. First, we measured the frequency of cells carr...

Biological interactions between human cytomegalovirus (HCMV) and the human immunodeficiency virus type 1 (HIV-1) were analysed in transfection and infection experiments, carried out in a human osteogenic sarcoma cell line (HOS) and in the same cell line chronically infected with HCMV (E155). When HOS and E155 cells were transfected with recombinant plasmids containing the HIV long terminal repe...

DNA marker is commonly used to determine the size of fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure prepare recombinant plasmids pSY-60 which was partially digested one restriction enzyme for generating markers 7 from 60 420 bp. The included synthesis bp fragment with EcoRI sites at both ends using PCR extension, self-ligation an...

BACKGROUND Severe and fatal complications of toxoplasmosis urge development of effective vaccines against the disease. The current study was performed to evaluate cocktail DNA vaccine containing plasmids encoding GRA5, SAG1, and ROP2 genes of Toxoplasma gondii in BALB/c mice in Tarbiat Modares University in 2012. METHODS The plasmids containing complete GRA5, SAG1, and ROP2 genes were mass ex...

background: the critical role of interleukin-7 (il-7) in homeostatic proliferation and t cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. methods: in the present study we expressed recombinant human interleukin-7 (rhil-7) in chinese hamster ovary (cho)-k1 cells. cho-k1 cells were stably transfected wit...