low- temperature (lt) tolerance is an economic trait in winter wheat that determines the plants’ ability to successfully cope with below freezing temperatures. to identify the genomic regions associated with lt tolerance in hexaploid wheat, an f2:3 population was produced from crossing between winter type and tolerant parent, mirnovoskaja 808 (lt50 = -20oc), and spring type and susceptible pare...

Urtica dioica is an important medicinal plant which is widely distributed in Mazandaran province (North of Iran). In this study for the first time Amplified Fragment Length Polymorphism (AFLP) and Inter-simple Sequence Repeat (ISSR) markers were used for detection of genetic polymorphism in Mazandaran nettle. Ten AFLP primer combinations and seventeen ISSR markers were utilized. AFLP produced 830...

The transcription unit of the 5C actin gene exhibits a complex organization that is unique among the six actin genes of Drosophila melanogaster. Three different mRNA size classes showing distinct patterns of accumulation throughout development are detected on Northern blots. We have determined the structure of the various 5C actin transcripts by exon mapping using strand-specific RNA probes, pr...

Catfish is the major aquaculture species in the United States. The hybrid catfish produced by crossing channel catfish females with blue catfish males exhibit a number of desirable production traits, but their mass production has been difficult. To introduce desirable genes from blue catfish into channel catfish through introgression, a genetic linkage map is helpful. In this project, a genetic...

Cfr and RlmN methyltransferases both modify adenine 2503 in 23S rRNA (Escherichia coli numbering). RlmN methylates position C2 of adenine while Cfr methylates position C8, and to a lesser extent C2, conferring antibiotic resistance to peptidyl transferase inhibitors. Cfr and RlmN show high sequence homology and may be evolutionarily linked to a common ancestor. To explore their individual speci...

Two cytologically stable wheat-Dasypyrum breviarisatatum addition lines, Y93-1-6-6 and Y93-1-A6-4, were identified by integrated molecular and cytogenetic techniques. C-banding and genomic in situ hybridization (GISH) showed that Y93-1-6-6 and Y93-1-A6-4 were different wheat-D. breviaristatum additions. A total of 51 markers (primer/enzyme combinations), including 6 PCR-based Landmark Unique Ge...

Amplified fragment length polymorphisms (AFLPs), along with some microsatellite and Type I markers, were used for linkage analysis in Crassostrea virginica Gmelin, the eastern oyster. Seventeen AFLP primer combinations were selected for linkage analysis with two parents and their 81 progeny. The 17 primer combinations produced 396 polymorphic markers, and 282 of them were segregating in the two...

Genetic diversity of six populations of bartail flathead (Platycephalus indicus Linnaeus, 1758) was investigated using amplified fragment length polymorphism (AFLP). A total of 118 reproducible bands amplified with ten AFLP primer combinations were obtained from 42 fishes that were collected from six different locations in the northern part of the Persian Gulf. The percentage of polymorphic ban...

In this study, SRAP molecular maker approach was performed to investigate genetic diversity in the Salvia genus. A total of 205 DNA bands were produced from PCR amplification of 11 Salvia species and populations using 25 selective primer combinations, of which 204 polymorphic genetic loci accounted. The total number of amplified fragments ranged from 3 to 15. The genetic similarities of 11 coll...

of high-quality vegetable and industrial oil. 9 RAPD primers, 6 AFLP primer combinations and 12 agro-morphological traits were used to assess the genetic diversity of 20 accessions of saf flower representing global germplasm variability. Jacquards’ similarity coef ficient were used to understanding the genetic relationships among ccessions, for AFLP and RAPD markers and Euclidian similarity coe...