background and aims: adventitious agents, especially viral agents are among the most important concerns in viral vaccines. viral contamination of biological products may arise from the original source of the reagents such as, serum, trypsin, animal or human derived media components or cell culture and working seed or cross contamination of vaccine during production. in this study, adventitious ...

one of the main problems in cell culture is mycoplasma infection. it can extensively affect cell physiology and metabolism. as the applications of cell culture increase in research, industrial production and cell therapy, more concerns about mycoplasma contamination and detection will arise. this review will provide valuable information about: 1. the ways in which cells are contaminated and the...

One of the limitations of conventional tissue culture on flat two-dimensional surfaces is the loss of complex interactions between the epithelium and stroma. We have devised a culture system that recreates the salient features of the stratified epithelium using primary cell cultures from mouse models. The protocol described here is applicable to the esophageal epithelium, but stratified epithel...

background and objectives: alzheimer's disease (ad) as a neurodegenerative disorder is the most common form of dementia in the elderly. according to the amyloid hypothesis, accumulation of amyloid beta (aβ) plaques, which are mostly constituted of aβ peptide aggregates, triggers pathological cascades that lead to neuronal cell death. thus, modulation of aβ toxicity is the hopeful therapeut...

objective: the aim of the present study was to investigate the neuroprotective effects of melissa officinalis, a major antioxidant plant, against neuron toxicity in hippocampal primary culture induced by 3,4-methylenedioxymethamphetamine (mdma) or ecstasy, one of the most abused drugs, which causes neurotoxicity. materials and methods: 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-tetrazolium bromid...

background: chlamydia trachomatis is the most reported bacterial sexually transmitted disease, especially among young women worldwide. aim of this study was comparison to evaluate the prevalence of chlamydia trachomatis infection in woman with tubal infertility by means of pcr and cell culture techniques. methods: fifty-one women with confirmed tfi were enrolled in this study in (avicenna infer...