The exact role of dopamine transporter gene (DAT1) in the pathogenesis of bipolar disorder type 1 (BD) is not understood. In the present study, we aimed to evaluate the possible association between 30, 40 and 63 bp variable number tandem repeat (VNTR) polymorphisms of DAT1 gene and the risk of type 1 (BD) in a sample of Iranian population. This case-control study was performed on 152 BD patient...

Background: Human brucellosis is a zoonotic disease caused by Brucella melitensis, Brucella abortus, and Brucella suis. Brucella causes a chronic disease, which subverts the immune defense system of their hosts. In this study, the prevalence of an important Brucella virulence determinant, PrpA, which can modulate immune response, was determined in human isolates. Methods: Polymerase chain reac...

Advances in biotechnology has been the subject of praise for a decade now, this is due to techniques such as gene expression which contributed immensely success genetic engineering, medical advancement, vaccine production and agriculture. Gene become very important tool overall improvement quality life. This paper tries look into development last forty years highlight how technological advancem...

Dictyophora rubrovolvata is an important edible mushroom that widely cultivated in China. In 2019, a serious rot disease on D. was observed production facility located Ce Heng County, Southwest of Guizhou Province, The causal agent identified as Trichoderma koningiopsis by amplification and sequencing the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (EF-1?) ge...

In this study, the relationship between IL-1β [-31 C / T] gene polymorphisms in herniated lumbar disc is offered to be investigated. was amplified by PCR [Polymerase Chain Reaction] method. Polymorphic site cut with restriction enzyme Alu I and genotyping were performed. Fifteen subjects (15%) homozygous for C/C allele at -31 position of gene. Of remaining 85 subjects, 64 (64%) heterozygous C/T...

Aim . To assess the contribution of polymorphic variants inflammatory response genes to predisposition rheumatic heart disease. Material and methods Using real-time polymerase chain reaction, we analyzed prevalence 18 8 involved in process 251 patients with disease 300 healthy donors. Results We found that homozygous TT genotypes rs1800871 (IL10) (p=0,02) rs1800872 polymorphisms (p=0,027), as w...

The CYP2A6 gene, which codes the enzyme, has known to have ahigh polymorphism. This polymorphism could decrease, increase, or eliminate enzyme activity. CYP2A6*4 , an inactive allele, decreased One of enzyme-specific substrates is nicotine. allele decrease nicotine metabolism that causes high levels in blood. In addition, it caused increasing Low-Density Lipoprotein Cholesterol (LDL-C) by expan...

The DNA polymerase gene of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) was cloned and sequenced. The predicted DNA polymerase protein (1113 amino acids, 115.9K) was found to have an amino acid identity of 48% with the corresponding gene of the Autographa californica MNPV (AcMNPV). It contains five domains associated with substrate binding, primase interaction, and...

The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized byreverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coatprotein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibodysandwich enzyme-linked immunesorbent assay (DASELISA) in a population ...

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was performed to investigate the allele frequencies of the insulin-like growth factor (IGF1 and IGF2 genes) in 90 broilerchickens. A 793 bp fragment of IGF1 gene and 1146 bp fragment of IGF2 gene were amplified and digested with HinfI and Hsp92II restriction enzymes,respectively. Two types of alleles of A and B...