نتایج جستجو برای: polya pcr
تعداد نتایج: 175977 فیلتر نتایج به سال:
Tujuan dari riset ini, yaitu melihat kompetensi peserta didik saat memecahkan permasalahan matematis sesuai tahapan Polya. Penelitian ini dilaksanakan ketentuan penelitian kualitatif deskriptif dimana sebanyak tiga tujuh belas orang siswa kelas VII pada salah satu SMP swasta di Kabupaten Karawang dipilih dengan teknik purposive sample atau berdasarkan kategori, yakni masing-masing kemampuan pem...
Background Classification of cancer subtypes by means of microarray signatures is becoming increasingly difficult to ignore as a potential to transform pathological diagnosis nonetheless, measurement of Indicator genes in routine practice appears to be arduous. In a preceding published study, we utilized real-time PCR measurement of Indicator genes in acute lymphoid leukaemia (ALL) and acute m...
Problem-solving ability is an important part of learning mathematics. The low to solve mathematics needs get attention related students' self-efficacy. This study aims determine the level mathematical problem-solving abilities based on categorization self-efficacy at high, medium, and levels. research was conducted in class XI SMAN 1 Konawe Utara with a total 37 students as subjects. type descr...
Abstract. We prove Poincaré and Plancherel–Polya inequalities for weighted p-spaces on weighted graphs in which the constants are explicitly expressed in terms of some geometric characteristics of a graph. We use a Poincaré-type inequality to obtain some new relations between geometric and spectral properties of the combinatorial Laplace operator. Several well-known graphs are considered to dem...
BACKGROUND Classification of cancer subtypes by means of microarray signatures is becoming increasingly difficult to ignore as a potential to transform pathological diagnosis; nonetheless, measurement of Indicator genes in routine practice appears to be arduous. In a preceding published study, we utilized real-time PCR measurement of Indicator genes in acute lymphoid leukaemia (ALL) and acute m...
We report an improved subtractive cDNA cloning procedure, named "directional tag PCR subtraction," for isolating clones of mRNAs enriched in a target tissue compared to a second tissue, the driver. In this method, the target and driver are prepared from directional cDNA libraries constructed in different vectors, and the target cDNA contains tag sequences at both its 5' and 3' ends for PCR ampl...
Gene expression analyses using spotted cDNA microarrays typically require relatively large quantities of total RNA (up to 100 microg) or polyA+RNA (1-5 microg). However, samples obtained by microdissection, patient biopsies, or embryonic samples often are small and yield an insufficient amount of RNA. Methods such as linear RNA amplification by in vitro transcription (IVT) or cDNA amplification...
The problem of estimating a unimodal density and its mode has attracted a wide interest in the literature, beginning with the work of Barlow [1], Prakasa Rao, [7], Robertson, [8], and Wegman [13], [14] and continuing through [9], [2], [3], [5], and [4], who can be consulted for further references. Asymptotic properties of the maximum likelihood estimators have been developed but may be messy an...
PURPOSE This study examined whether retinal pigment epithelial (RPE) cells have the capacity to express transforming growth factor-beta (TGF-beta). Also examined were TGF-beta isoform genes expressed in the RPE cells. METHODS Complementary DNA (cDNA) was generated from polyA+ RNA extracted from human RPE cells in culture, and polymerase chain reaction (PCR) using degenerate and specific prime...
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