The extent of conservation in the programmed cell death pathways that are activated in species belonging to different kingdoms is not clear. Caspases are key components of animal apoptosis; caspase activities are detected in both animal and plant cells. Yet, while animals have caspase genes, plants do not have orthologous sequences in their genomes. It is 10 years since the first caspase activi...

By using biochemical, immunological, and molecular strategies we have identified and cloned a cDNA encoding a protease from tomato (Lycopersicon esculentum) plants (P69B) that is part of a proteolytic system activated in the plant as a result of infection with citrus exocortis viroid. This new protease is closely related, in terms of amino acid sequence and structural organization, to the previ...

Aim: The present study aims at identifying potential inhibitors from a set of ten compounds Ocimum basilicum against the SARS-CoV-2 main protease, chymotrypsin-like protease (3CLpro).
 Materials and Methods: Computational studies by molecular docking (Autodock tool) were used to obtain scoring function phytochemicals in interaction with protease. pharmacokinetic behavior high-docking score...

Protease and virulence of the extracellular products (ECP) of Vibrio carchariae strain EmI82KL, a causative agent of gastroenteritis in Epinephelus coioides, cultured on different media were studied. The bacteria grown on peptone agar, nutrient agar or brain heart infusion agar produced higher protease activities than that grown on tryptic soy agar (TSA) in terms of protein content. The additio...

The general potential of plant cystatins for the development of insect-resistant transgenic plants still remains to be established given the natural ability of several insects to compensate for the loss of digestive cysteine protease activities. Here we assessed the potential of cystatins for the development of banana lines resistant to the banana weevil Cosmopolites sordidus, a major pest of b...

Proteases, though essentially indispensable to the maintenance and survival of their host organisms, can be potentially damaging when overexpressed or present in higher concentrations, and their activities need to be correctly regulated. An important means of regulation involves modulation of their activities through interaction with substances, mostly proteins, called protease inhibitors. Some...

Proteases catalyze the hydrolysis of a peptide bond of a protein; they are widely distributed in nature. They perform various biological functions in plants and particularly in humans they participate in zymogen activation, fibrinolysis, complement activation, blood coagulation and digestion process. Objectives: The present study focused on evaluation of in vitro total proteolytic activity from...

Internet Electronic Conference of Molecular Design 2003, November 23 – December 6 Abstract This article describes a Traditional Chinese Medicine Plant-Compound Database and an application case of database searching for HIV protease inhibitor. It offers not only basic compound properties such as English names and synonyms, physical properties, natural sources, bioactivity data, formula, molecula...

Positive selection is thought to contribute to the functional diversification of insect-inducible protease inhibitors in plants in response to selective pressures exerted by the digestive proteases of their herbivorous enemies. Here we assessed whether a reciprocal evolutionary process takes place on the insect side, and whether ingestion of a positively selected plant inhibitor may translate i...

The ATP-dependent Clp protease in plant chloroplasts consists of a heterogeneous proteolytic core containing multiple ClpP and ClpR paralogues. In this study, we have examined in detail the only viable knockout mutant to date of one of these subunits in Arabidopsis thaliana, ClpR1. Loss of ClpR1 caused a slow-growth phenotype, with chlorotic leaves during early development that later partially ...