Latex allergy is recognized worldwide as a serious health risk. To date, exposure assessment and intervention strategies have focused primarily on respiratory protection; this work evaluates the potential role of dermal protein penetration in the development of latex allergy. In vitro penetration models using flow-through diffusion cells and both human surgical specimens and hairless guinea pig...

The major proteoglycan of pig skin, a proteodermatan sulfate, has been isolated under mild conditions in the presence of protease inhibitors. After purification by ion exchange and gel chromatography, it has a Mr of 70 x 10(3) and it does not form aggregates with hyaluronic acid. It contains about 60% protein, one chain of dermatan sulfate, and several oligosaccharide chains. The dermatan sulfa...

We examined the intradermal delivery of a hydrophilic polyphenol chlorogenic acid by in vitro study using excised guinea pig dorsal skin and Yucatan micropig skin. Skin accumulation as well as the solubility of chlorogenic acid in aqueous vehicles was much greater than for other polyphenols such as quercetin and genistein. However, since enhancement of skin delivery seemed to be necessary to ex...

BACKGROUND Scabies is a contagious skin disease rarely described in miniature pigs. To the best of the authors' knowledge, a zoonotic transfer from infected pet pigs to humans has not been reported previously. CASE PRESENTATION This case report describes the infestation with Sarcoptes scabiei mites in two miniature pigs presenting with unusual clinical signs, and disease transmission to a chi...

Intradermal administration of naked DNA with a conventional needle syringe is simple and inexpensive method to expose an encoded antigen the dermal immune system. We aimed enhance intradermal gene expression pyro-drive jet injector using pig skin, which similar in structure biomechanical properties human skin. When Cy3-labeled plasmid (pCy3) was applied skin injector, pCy3 distributed preferent...

Flavonoids are bioactive phytochemicals that exhibit protective potential against cutaneous inflammation and photoaging. We selected eight flavonoid aglycones or glycosides to elucidate the chemistry behind their skin absorption capability through experimental and computational approaches. The skin delivery was conducted using nude mouse and pig skins mounted on an in vitro Franz cell assembly....

We will report on crosslinking of ceramides molecules in the stratum corneum of a live pig by non-equilibrium atmospheric pressure asymmetric dielectric barrier discharge treatment. This crosslinking leads to formation of temporary pores in the skin, leading to increased permeability of the skin which we have validated with vitamin D.

The in vivo efficacy of the novel polymeric guanidine AKACID Plus was evaluated in a guinea pig model of experimental skin infection with methicillin-resistant Staphylococcus aureus (MRSA). Topical application of AKACID Plus at concentrations of > or =0.5% was as effective as mupirocin 2% cream in the treatment of superficial skin infection with MRSA.

Hypersensitivity responses to two highly purified and well characterized mycobacterial proteins and a preparation of mycobacterial arabinogalactanarabinomannan were studied by using guinea pig skin tests and in vitro-cultured human lymphocyte mitogenesis stimulation assays. The purified proteins were found to elicit delayed-type hypersensitivity skin test reactions and to stimulate mitogenesis ...