the aim of the study was to determine the resistance patterns of pseudomonas aeruginosa isolates recovered from patients with surgical wounds in hospitals and also to investigate their epidemiological relatedness using molecular typing techniques. twenty pseudomonas sp. isolated from surgical wounds were subjected to antibiotic susceptibility testing by disk diffusion, plasmid profile, sds-page...

The deteriorating effect of corrosion can be controlled by applying suitable polymeric-based coatings. In this work, polyolefin based smart composite coatings containing modified halloysite nanotubes decorated with ceria particles were investigated to analyze their anti-corrosion behavior. For purpose, (Hals) utilized as nanocarriers which loaded sodium dodecyl sulfate (SDS) a inhibitor via ove...

results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...

Rochalimaea quintana was isolated from the blood of a French human immunodeficiency virus-infected patient with bacillary angiomatosis. The isolate showed the typical growth characteristics of Rochalimaea species and was inert when typical biochemical testing was used. The purpose of the present work was to characterize and compare this new isolate with reference strains of R. quintana, Rochali...

The Andean weevil Premnotrypes vorax represents an important cause of damage to Colombian potato crops. Due to the impact of this plague on the economy of the country, we searched for new alternatives for its biological control, based on the entomopathogenic bacteria Bacillus thuringiensis. A total of 300 B. thuringiensis strains obtained from potato plantations infested with P. vorax were anal...

BACKGROUND DNA extraction is a routine step in many insect molecular studies. A variety of methods have been used to isolate DNA molecules from insects, and many commercial kits are available. Extraction methods need to be evaluated for their efficiency, cost, and side effects such as DNA degradation during extraction. METHODOLOGY/PRINCIPAL FINDINGS From individual western corn rootworm beetl...

Source/Description: The polymerase chain reaction (PCR) and allele specific oligonucleotide (ASO) hybridisation were used to detect a common polymorphism within the open reading frame (ORF) of the human prion protein (PRIP) gene. This polymorphism has previously been reported as a mutation (1). The oligonucleotide sequences for amplification of the ORF of the PRIP contained EcoRI and BamHI link...