An integrated continuous-flow microfluidic chip was fabricated on glass substrate with PDMS based microchannels, cell lysis and PCR modules. Gold-microelectrodes were used to produce electrochemical cell lysis, while, indium-tin-oxide (ITO) microheater was used for thermal cycling of PCR reaction. The fabricated device was used for 20 cycles of PCR amplification of pancreatic cancer DNA marker ...

PURPOSE To study the applicability (sensitivity, specificity) of polymerase chain reaction (PCR) tests in the detection of cytomegalovirus (CMV), herpes virus (HSV) and varicella zoster (VZV), Epstein-Barr virus (EBV), Mycobacterium sp and Toxoplasma gondii in the diagnosis of patients with or without AIDS, with presumably infectious uveitis, using serum, aqueous humor and vitreous humor sample...

Objective : The study aimed to evaluate molecular and immunological methods propose a workflow using them for tuberculosis (TB) diagnosis routine. Methods A cross-sectional retrospective was performed, including 121 liquid cultures from TB laboratory located in the extreme south of Brazil. All were positive Mycobacterium complex (MTBC) by in-house Polymerase Chain Reaction (PCR) DNA extracted C...

Calreticulin (CALR) mutations are included in the WHO diagnostic criteria of Myeloproliferative Neoplasm (MPN). Presence CALR mutation, aids their diagnosis by excluding reactive causes. There several molecular techniques to detect mutations; however, these require advanced equipment and technical expertise. An in-house “polymerase chain reaction-restriction fragment length polymorphism (PCR- R...

In polymerase chain reaction (PCR) product analysis, a comparison of target amplicon yield to such PCR artifacts as primer-dimers and misprimed secondary products is essential in PCR assay optimization. This process requires accurate and reproducible measurement of amplified DNA products over a wide range of sizes and concentrations. The 2100 Bioanalyzer is ideally suited to provide rapid quant...

Thesis Abstract Several cytokines are known to be involved in the pathogenesis of non-Hodgkin Lymphomas (NHL). Given importance understanding genetic predisposition NHL, this work was designed study impact IL-10 (-1082 G/A; rs1800896 and -819 C/T; rs1800871), IL-6 (-174 G/C; rs1800795) CD38 (184C/G; rs6449182) gene polymorphisms on susceptibility Egyptians NHL. Both loci IL10 were genotyped usi...

PCR, including real-time PCR, usually requires at least 1 h to obtain results. To shorten this time, a novel real-time PCR method (Hyper-PCR) was developed. This method utilizes high-speed DNA polymerase and a thin disc-type reaction vessel that can quickly alter the temperature of the reaction mixture in a newly developed PCR machine. Reactions capable of amplifying adenovirus (Ad) DNA can be ...

objective: polymerase chain reaction (pcr) is one of the most important progress in the field of molecular biology and diagnosis. despite simplicity in concept, the reaction needs complex interaction between target sequence, primers, dntps and dna polymerase for a successful amplification and diagnosis. for the detection of rna viruses highly sensetive and specific technique is required. hence ...

Polymerase chain reaction (PCR) targeting human beta-globin gene has been reported to be able to detect micrometastasis in an animal model. We attempted to detect cancer cells from the lung and peripheral blood in nude mice, into which a human adenoid cystic carcinoma (ACC) line (KOA-1 and KOA-1L3) had been transplanted. Positive PCR reaction was observed in the lung of a total of 19 of 47 (40....