This investigation demonstrates the status of bovine anaplasmosis caused by A. marginale in bovines from Submountain and Undulating Zone of Punjab. Out of 184 suspected animals, 25 (19.51%), 47 (31.71%), and 78 (68.75%) were positive by microscopy, indirect ELISA, and PCR assay, respectively. The microscopy showed 29% sensitivity and 99% specificity, while ELISA showed 32% sensitivity and 79% s...

Abstract Canine visceral leishmaniasis (CVL) caused by Leishmania (Leishmania) infantum is transmitted phlebotomine sandflies and a major zoonotic disease in Brazil. Due to the southward expansion of within country central role dogs as urban reservoirs parasite, we have investigated occurrence CVL two municipalities Erval Velho Herval d’Oeste Midwest region Santa Catarina state. Peripheral bloo...

Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants.  Objective: The main goal of the present study was to eva...

With the objective of identifying presence Paratuberculosis (PTB), an infectious disease caused by Mycobacterium avium ssp paratuberculosis (MAP), in sheep, through pathological studies, bacterial culture and IS900 PCR, as well estimating seroprevalence to MAP. The present cross-sectional study, was conducted 16 different flocks, with serum 2415 adult sheeps, analyzed Enzyme-Linked ImmunoSorben...

Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high speci...

The exopolysaccharide secreted by Lacticaseibacillus paracasei subsp. SS-01 strain (LP-EPS) is isolated and purified from yogurt. It a polysaccharide with branched multi-stranded structure, which exists in smooth rod-like or cloud-like state, possesses good thermal stability molecular weight of 49.68 kDa (±4.436%). LP-EPS shows high antioxidant capacity, anti-inflammatory anti-sensitizing activ...

BACKGROUND We aimed to develop a PCR-RFLP assay based on available sequences of putative vertebrate hosts to identify blood meals ingested by field female sand fly in the northwest of Iran. In addition, the utility of PCR-RFLP was compared with ELISA as a standard method. METHODS This experimental study was performed in the Insect Molecular Biology Laboratory of School of Public Health, Tehra...

The study have been done to detecting HRSV infections among hospitalized children under 10 years old in Salahaldin Governorate, addition compare different diagnostic methods for detection of this virus.
 Nasopharyngeal Swabs (NPS) and whole blood samples were taken from 400 between end August 2013, the May 2014, each sample was analyzed by Real time –PCR, IgM IgG specific determining speci...