Demineralised homologous bone-matrix implant was used to bridge a large circumferential osteoperiosteal gap in the diaphysis of the ulna of rabbits. Periodic observations of the graft were made clinically, radiologically, histologically and by tetracycline fluorescence up to forty-two weeks. By the twelfth week after operation 81 per cent of the animals revealed bone formation in the implant an...

Autologous bone grafting remains the gold standard for bone grafting in clinical practice. Although it has withstood the test of time, it remains associated with multiple comorbidities. The search for an alternative bone graft substitute harnessing bone's osteoconductive, osteoinductive, and osteogenic properties remains a challenge. This article examines the various bone grafting materials cur...

A novel bone implant material containing biphasic calcium phosphate (BCP), casein (CA), hen egg yolk (EY) and the extracts of Ormocarpum sennoides (Os) plant was prepared. It has shown porosity, biocompatibility, cytocompatibility and better alkaline phosphatase activity that is required for osteoinduction. In vitro studies using SaOs-2 cell line have exhibited the osteoinductive nature of the ...

Klotho is a potent regulator of 1,25-hydroxyvitamin D3 [1,25(OH)2D3] formation and calcium-phosphate metabolism. Klotho-hypomorphic mice (kl/kl mice) suffer from severe growth deficits, rapid aging, hyperphosphatemia, hyperaldosteronism, and extensive vascular and soft tissue calcification. Sequelae of klotho deficiency are similar to those of end-stage renal disease. We show here that the mine...

The aim of this study was to investigate physical and biological properties of collagen (COL) and demineralized bone powder (DBP) scaffolds for bone tissue engineering. DBP was prepared and divided into three groups, based on various particle sizes: 75-125 µm, 125-250 µm, and 250-500 µm. DBP was homogeneously mixed with type I collagen and three-dimensional scaffolds were constructed, applying ...

PURPOSE Nowadays, research in magnesium alloys as a biodegradable implant material has increased. The aim of this study was to examine osteoinductive properties and tissue responses to pure magnesium in comparison to conventional permanent (titanium) and to degradable (glyconate) implant materials. METHODS Magnesium wires (0.4 mm in diameter, 10 mm length) were implanted into tail veins of mi...

Numerous strategies that are currently used to regenerate bone depend on employing biocompatible materials exhibiting a scaffold structure. These scaffolds can be manufactured containing particular active compounds, such as hydroxyapatite precursors and/or different growth factors to enhance bone regeneration process. Herein, we have immobilized calcium phosphate salts (CPS) and bone morphogene...

Bone morphogenetic proteins (BMP) have osteoinductive activity and therefore play important roles in overcoming bone disorders. The use of the graphene-based scaffolds in bone tissue engineering appears as a good alternative for the autologous and allogeneic bone grafts, metal alloys or ceramics, which are widely used in case of bone injuries. The aim of the present study was to provide a detai...

Bone callus, generated during fracture healing, is commonly discarded during surgical procedures. The aim of this study was to investigate the osteogenic potential of bone callus and its possible use as autograft material for patients needing bone grafts. Histology, immunohistochemistry, micro-computed tomography, and biomechanics were performed to examine osteogenic cells, osteoinductive facto...

The natural pure compound obtusilactone A (OA) was identified in Cinnamomum kotoense Kanehira & Sasaki, and shows effective anti-cancer activity. We studied the effect of OA on osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). OA possesses biocompatibility, stimulates Alkaline Phosphatase (ALP) activity and facilitates mineralization of BMSCs. Expression of osteogenesis ma...