This method is suitable for the determination of bromate residues in a variety of baked goods. The peer-verified method trial was performed on white bread, multigrain bread, and coffee cake spiked with known levels of potassium bromate. The analytical portion is extracted with deionized water to remove bromate from the bulk of the baked product. The aqueous extract is carried through a series o...

BACKGROUND AND OBJECTIVE Due to the evolution of multidrug-resistant strains, screening of natural resources, especially actinomycetes, for new therapeutic agents discovery has become the interests of researchers. In this study, molecular, chemical and biological screening of soil actinomycetes was carried out in order to search for peptide-producing actinomycetes. MATERIALS AND METHODS 60 ac...

-1. Phlorizin hydrolase activity has been determined in the intestinal homogenates of ten species. The activity decreased in the following order: frog, rabbit, squirrel, rat and monkey. The activity was either very low or could not be detected in chicken, pigeon, guinea-pig, goat and human. 2. The enzyme was optimally active in the pH range 5.0-5.7 in all the species investigated except in the ...

Polyacrylamide gel electrophoresis (PAGE) is a common technique used in Biochemistry for separation of macromolecules [I]. It is particularly useful to ascertain the degree of protein purity and confirm molecular masses obtained from chromatographic methods [2-51. In addition PAGE can be developed as zymogram (native gel) to detect the presence of enzyme activity [6]. In this report we describe...

BACKGROUND Mutations in the gene ATP7B cause Wilson disease, a copper storage disorder with a high phenotypic and genetic heterogeneity. We aimed to evaluate whether 'severe' protein-truncating ATP7B mutations (SMs) are associated with low serum ceruloplasmin oxidase activities and an early age of onset when compared to missense mutations (MMs). METHODS The clinical phenotype of 59 geneticall...

MicroRNA-145 (miR-145) is considered to play key role in many cellular processes, such as proliferation, differentiation and apoptosis by inhibiting target gene expression. The transcriptional regulation of cardiovascular development requires precise spatiotemporal control of gene expression. We are beginning to understand the functions of miRNA-145 played during essential biological processes ...

Superoxide dismutases (SODs) catalyze the dismutation of superoxide radicals in a broad range of organisms, including plants. Quantification of SOD activity in crude plant extracts has been problematic due to the presence of compounds that interfere with the dose-response of the assay. Although strategies exist to partially purify SODs from plant extracts, the requirement for purification limit...

The insulin-like growth factor (IGF) family is essential for normal embryonic growth and development and it is highly conserved through vertebrate evolution. However, the roles that the individual members of the IGF family play in embryonic development have not been fully elucidated. This study focuses on the role of IGF-2 in zebrafish embryonic development. Two igf-2 genes, igf-2a and igf-2b, ...

Catalase-peroxidases (KatGs) are unique peroxidases exhibiting a high catalase activity and a peroxidase activity with a wide range of artificial electron donors. Exchange of tyrosine 249 in Synechocystis KatG, a distal side residue found in all as yet sequenced KatGs, had dramatic consequences on the bifunctional activity and the spectral features of the redox intermediate compound II. The Y24...

We report here the use of multiple indicators for a single analyte for paper-based microfluidic devices (microPAD) in an effort to improve the ability to visually discriminate between analyte concentrations. In existing microPADs, a single dye system is used for the measurement of a single analyte. In our approach, devices are designed to simultaneously quantify analytes using multiple indicato...