Dengue virus that causes dengue fever and shock syndrome has 4 different serotypes. Serotyping is needed for diagnosing surveillance activities of disease spreaders. Recently, the Nucleic Acid Lateral Flow (NALF) method been developed to confirm results easy amplification without complicated equipment. The aim this study was designing capture probe serotyping (DENV) using NALF method. We have c...

Designs and applications of microfluidics-based devices for molecular diagnostics (Nucleic Acid Amplification Tests, NAATs) in infectious disease testing are reviewed, with emphasis on minimally instrumented, point-of-care (POC) tests for resource-limited settings. Microfluidic cartridges ('chips') that combine solid-phase nucleic acid extraction; isothermal enzymatic nucleic acid amplification...

Nucleic acid amplification is a gold standard technique for analyzing a tiny amount of nucleotides in molecular biology, clinical diagnostics, food safety, and environmental testing. Electrical and electrochemical monitoring of the amplification process draws attention over conventional optical methods because of the amenability toward point-of-care applications as there is a growing demand for...

Biosensors have shown great potential in realizing rapid, low cost, and portable on-site detection for diseases. This work reports the development of a new bioelectronic sensor called AC electrokinetics-based capacitive (ABC) biosensor, genomic DNA (gDNA) methicillin-resistant Staphylococcus aureus (MRSA). The ABC is based on interdigitated microelectrodes biofunctionalized with oligonucleotide...