due to the worldwide increase in the number of older people in both developed and developing countries, there is a public health concern for dealing with age related diseases such as neurodegenerative diseases. there is little known about the difference in neuronal cell responses between genders. our understanding of the neuronal cell response regarding genders, will be useful for developing mo...

Astrocytes, due to the proximity to neuronal lineage and capability to proliferate, are ideal starting cells to regenerate neurons. Human fetal astrocytes have been successfully converted into neuronal cells by small molecules, which offered a broader range of further applications than transcription factor-mediated neuronal reprogramming. Here we report that human adult astrocytes could also be...

The differentiation of neural cells from embryonic stem cells is influenced by several growth factors. Amniotic epithelial cells (AECs) share many of the same characteristics as embryonic stem cells, and therefore those factors may similarly affect the derivation of neural cells from AECs. In this study, we examined the differentiation of neural cells in vitro from AECs following AECs treatment...

NRXN1 encodes thousands of splicing variants categorized into long NRXN1α, short NRXN1β and extremely NRXN1γ, which exert differential roles in neuronal excitation/inhibition. NRXN1α deletions are common autism spectrum disorder (ASD) other neurodevelopmental/neuropsychiatric disorders. We derived induced pluripotent stem cells (iPSCs) from one sibling control two ASD probands carrying NRXN1α+/...

We characterized phenotype and function of a fetal human mesencephalic cell line (LUHMES, Lund human mesencephalic) as neuronal model system. Neurodevelopmental profiling of the proliferation stage (d0, day 0) of these conditionally-immortalized cells revealed neuronal features, expressed simultaneously with some early neuroblast and stem cell markers. An optimized 2-step differentiation proced...

Bisphenol A (BPA) is an extremely common polymer that is used in typical everyday products throughout the world, especially in food and beverage containers. Within the last ten years, it has been found that the BPA monomer tends to leach into foodstuffs, and nanogram concentrations of it may cause a variety of deleterious health effects. These health problems are very evident in developing chil...

Achieving safe and readily accessible sources for cell replacement therapy in Parkinson's disease (PD) is still a challenging unresolved issue. Recently, a primitive neural crest stem cell population (hOMSC) was isolated from the adult human oral mucosa and characterized in vitro and in vivo. In this study we assessed hOMSC ability to differentiate into dopamine-secreting cells with a neuronal-...

endometriosis is a common gynaecological disease that can cause severe pelvic pain such as dysmenorrhoea and dyspareunia, however the mechanisms by which pain is generated are not well understood. nerve fibres in endometriotic plaques have been reported by several authors. we have recently demonstrated the presence of unmyelinated sensory nerve fibres (using the pan-neuronal marker pgp9.5) in t...

RNAscope® ISH technology provides a unique detection method for neuronal and glial cells markers that is both highly sensitive and specific for detection of any gene expressed in any neuronal and glial cell1. Because of its proprietary “double Z” probe, RNAscope® technology is a powerful tool to detect any gene, especially low abundance targets. And not only can RNAscope® technology be used to ...