نتایج جستجو برای: nanodrop
تعداد نتایج: 165 فیلتر نتایج به سال:
In the original published version of this article, text “Immediately after plasma exposure, cells were washed with PBS and dissociated using Accutase® (Innovative Cell Technologies, Inc., San Diego, CA, USA), counted, 1–2 × 106 plated in a 10-cm non-coated dish. Cells cultured for four days EB differentiation medium followed by three-day culture consisted 80% DMEM (with glutamine non-essential ...
Circulating blood microparticles are submicron vesicles released primarily by megakaryocytes and platelets that act as transcellular communicators. Inflammatory conditions exhibit elevated blood microparticle numbers compared to healthy conditions. Direct functional consequences of microparticle composition, especially internal composition, on recipient cells are poorly understood. Our objectiv...
Polyhydroxyalcanoates (PHAs) are biodegradable polymers synthesized in cytoplasmic granules in bacteria, such as Cupriavidus necator (Ralstonia eutropha), Alcaligenes latus, Pseudomonas spp., Comamonas spp. and other species. PHAs accumulation occurs in response to stress conditions, i.e. under high carbon and low nitrogen (24:1 ratio). PHA can be synthesized using recombinant microorganisms (p...
Background: Isoberlinia (Craib and Stapf) is a genus with high economic pharmacological values. Aim: This study aimed at establishing the morphological, anatomical molecular characterisation of leaves I. doka tomentosa, which were conducted for proper authentication. Setting: The tomentosa obtained from Shika, kaduna State, Nigeria. Method: Morphological characters determined according to stand...
Aims: To successfully utilize non-invasive shed skin samples for species identification of captive snake Tamil Nadu and create genetic repository.
 Study Design: The experiment was designed to apply the ammonium acetate method DNA extraction from skin. Two mitochondrial markers were used ascertain species.
 Place Duration Study: Advanced Institute Wildlife Conservation (AIWC), Forest ...
the dna molecule has been known to be the cellular target for many cytotoxic anticancer agents for several decades. understanding how drug molecules interact with dna has become an active research area in the interface between chemistry, molecular biology and medicine. dna extraction has been suggested as a main step affecting molecular dna technology such as pcr and pcr-based methods. therefor...
We present the desolvation mechanism of a double-stranded oligodeoxynucleotide (dsDNA) from an aqueous nanodrop studied by using atomistic molecular dynamics methods. The central theme of this study is the stability of a non-covalently bound complex, in general, and that of a dsDNA in particular, in a droplet environment. Among the factors that may affect the stability of a complex in an evapor...
Introduction: IFN-γ is one of the most important cytokines in the development of chronic inflammatory diseases such as asthma and COPD. The IFN-γ promoter methylation at CpG islands could be altered by various chemical and toxic substances which may have a role in the incidence of chronic pulmonary diseases and its severity. Thereby, the present study was aimed to evaluate the effect of sulfur ...
AIMS Mutation detection accuracy has been described extensively; however, it is surprising that pre-PCR processing of formalin-fixed paraffin-embedded (FFPE) samples has not been systematically assessed in clinical context. We designed a RING trial to (i) investigate pre-PCR variability, (ii) correlate pre-PCR variation with EGFR/BRAF mutation testing accuracy and (iii) investigate causes for o...
Biofabrication of metallic nanoparticles have attracted the researchers of various disciplines for the past few decades, due to their wide range of applications in the various fields of electronics, textiles industries, nano medicine, biopharmacy and biotechnology. Keeping in view the above important applications, we have successfully biosynthesized the silver nanoparticles (AgNPs) using leaf e...
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