This study aimed to assess the effects of 17α-methyltestosterone (MT) on growth and reproductive performance male Asian catfish (Clarias macrocephalus). A total 36 immature (ABW = 125.97 g) were randomly distributed into 12 circular tanks, fed with either control diet (with no MT) or diets containing MT at 60 mg·kg-1, 90 120 mg·kg-1 for 45 days. Results showed that weight gain (WG), specific ra...

All-male tilapias are desirable because they manifest superior growth characteristics compared to females. The synthetic steroid, 17α-methyltestosterone (MT) has been commonly used to sex-reverse tilapia but because of its potential health and environmental hazards, the use of phytochemicals as alternative means to affect sex differentiation has to be explored. We addressed objective 1 by using...

Progesterone, 17-hydroxyprogesterone and four other 3-keto steroids were determined by high performance liquid chromatography with fluorescence detection. Each steroid was derivatized with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene++ +-3- propionohydrazide (BODIPY FL hydrazide) and separated on a Wakosil 5C4 column with acetonitrile-water (7 + 3) as mobile phase. The limits of dete...

An 81-year-old man, admitted with lumbago and pollakisuria, was diagnosed to have stage D2 prostate cancer (T3N2M1). Although the serum level of prostate specific antigen (PSA) was 3,560 ng/ml, serum testosterone (55.6 ng/dl) and luteinizing hormone (LH, 0.8 mIU/ml) levels were very low. Detailed inquiry of the patient revealed a daily intake of patent medicine containing 6-9 mg methyltestoster...

Infection of human foreskin cells (D-550) by the Snyder-Theilen strain of feline sarcoma virus produced small but countable foci and demonstrated "single-hit" dose-response kinetics. Significant quantitative and qualitative enhancement of focus formation was observed when the glucocorticoid hormones, dexamethasone, hydrocortisone, cortisol acetate, and prednisolone were added to cell cultures (...