4-Amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase catalyzes a complex rearrangement of 5-aminoimidazole ribonucleotide (AIR) to form HMP-P, the pyrimidine moiety of thiamine phosphate. We determined the three-dimensional structures of HMP-P synthase and its complexes with the product HMP-P and a substrate analog imidazole ribotide. The structure of HMP-P synthase reveals a h...

thi4 mutants of Schizosaccharomyces pombe exhibit defective thiamine biosynthesis, and thi4 mutations define a gene which is believed to be involved in the phosphorylation of 4-amino-5-hydroxymethyl-2-methylpyrimidine or 5-(2-hydroxyethyl)-4-methylthiazole and/or in the coupling of the two phosphorylated precursors to thiamine monophosphate (A. M. Schweingruber, J. Dlugonski, E. Edenharter, and...

• An anti-HIV heterocyclic compound BMMP to induce abnormal core uncoating. hnRNP M as a BMMP-binding protein. Anti-cell migration activity of BMMP. Function suppress CD44 mRNA expression via regulation the M. One sometimes shows two biological functions, becoming important aspect recent drug discovery. This study began with an attempt confirm previously reported molecular mechanism anti-human ...

Ultrasonic transmitters were surgically implanted into adult tautog (n=27, 400–514 mm TL) to document sea­ sonal occurrence and site utilization at four sites situated within known tautog habitat near Cape Charles, Virginia, in lower Chesapeake Bay. Tagged tautog were released at the same sites where originally caught within 2 h of capture. Sites were continuously monitored with automated acous...

Most medical treatment of postmenopausal osteoporosis are inhibitors of bone resorption and urinary deoxypyridinoline (D-Pyr) has been shown to be a reliable indicator of bone resorption. Fifty-one healthy women were divided into four groups. Group A: premenopausal women (n = 10), Group B: postmenopausal women, no osteoporosis, not on hormone replacement therapy (HRT) (n = 14), Group C: postmen...

Crystallographic data analyses indicate that  three types of prolinium cations, along with two types of hydrogen  bonding,  produce and stabilize the helical structure of  triprolinium 12-phosphomolybdate. There are similarities between this organic-inorganic compound and peptides/proteins. The stronger “conventional” hydrogen bonds and the less common C-H…O attractions play critical roles in g...

We report herein the development of a highly sensitive colorimetric method for the detection of 4-hydroxy-2-mercapto-6-methylpyrimidine (MTU) which acts as an anti-thyroid drug utilizing citrate capped gold nanoparticles (Au-NPs). This thiol-containing molecule exhibits intriguing affinity with Au-NPs. The reactivity involves the displacement of the citrate shell by the thiolate shell followed ...

The effect of a low dose of methyldopa combined with (a) a non-selective and (b) a selective beta-adrenoceptor antagonist was studied in a double-blind crossover trial in 24 carefully selected patients with moderate hypertension (mean initial lying blood pressure 189/117 mm Hg). Each patient received methyldopa 750 mg/day, propranolol 240 mg/day, practolol 600 mg/day, methyldopa 750 mg/day comb...

The 1:1 cocrystal of 2-amino-4-chloro-6methylpyrimidine (2A4C6MP) with 4-methylbenzoic acid (4MBA) (I) has been prepared by slow evaporation method in methanol, which was crystallized in monoclinic C2/c space group, Z = 8, and a = 28.431 (2) Å, b = 7.3098 (5) Å, c = 14.2622 (10) Å and β = 109.618 (3)°. The presence of unionized –COOH functional group in cocrystal I was identified both by spectr...