Background: Wharton’s jelly is an unlimited source of stem cells that can be used in cell therapy and tissue engineering without any ethical concern. It has been revealed the cell-free extract could be effective to induce cell differentiation. The objective of this study was to induce Wharton’s jelly-derived mesenchymal stem cells (MSCs) into hepatocyte-like cells by premeabilization of the cel...

introduction: chemokine receptors are found on the surface of stem cells. there have been 19 distinct chemokine receptors described in mammals. chemokines are major players in migration and homing. therefore, changes in their levels or function can help us to increase the migratory potential of these cells. valproic acid differs in structure from other drugs in common use. the way in which valp...

Mesenchymal stem cells (MSCs) have emerged as a promising tool for the treatment of Alzheimer’s disease (AD). Previous studies suggested that coculture human MSCs with AD in an vitro model reduced expression amyloid-beta 42 (A?42) medium well overexpression amyloid-beta- (A?-) degrading enzymes such neprilysin (NEP). We focused on role primed (human Wharton’s jelly-derived mesenchymal (WJ-MSCs)...

Hyaline cartilage is a vascular and neural tissue with scanty chondrocytes and limited regenerative ability. After some serious injuries of the cartilage, healing process will take place through the formation of fibrocartilage structures. Currently, tissue engineering and cell therapy are 2 interesting therapeutic fields dealing with regenerative medicine. In this regard, tissue&...

human umbilical cord matrix (hucm) is considered as a promising source of mesenchymal stem cells (mscs) due to several advantages over other tissues. the potential of neural differentiation of hucm-mscs is of great interest in the context of treating neurodegenerative diseases. in recent years, considerable efforts have been made to establish in vitro conditions for improving the differentiatio...

Background: Lead (Pb) is a heavy metal that has devastating effects on many animal tissues. In this study we investigated the effects of orally-dosed lead acetate II on osteocalcin gene (osteocalcin) expression in mesenchymal stem cells grown in an osteogenic medium. Osteocalcin is an abundant bone matrix differentiation protein. Methods: Twelve male Wistar rats were divided into three groups ...

purpose : our aim was to isolate mesenchymal stem cells (mscs) from mouse umbilical cord and culture them with vitreous body to investigate their capacity to differentiate into lens fiber cells, crystallins are expressed. methods : wharton's jelly is the appropriate source of mscs. after isolation, the cells were cultured and alkaline phosphatase detection kit was used for staining undiffe...

Background  Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...

Mesenchymal stem cells (MSCs) have the ability to differentiate into neuronal like cells under appropriate culture condition. In this study, we investigated whether MSCs derived from human peripheral blood (PB-MSCs) can differentiate into neuronal like cells by synergic effect of the growth factors EGF, bFGF and Noggin. For this purpose, the expression of five neuronal markers (Nestin, &beta II...

background bone marrow and adipose tissue are two main sources of mesenchymal stem cells (mscs). some of studies suggest that there are some differences in gene expression profile of mscs-derived from various tissues. to investigate gene expression profile by qrt-pcr, an appropriate reference gene with stable expression level should be chosen for normalizing data.  this study was designed to ev...