نتایج جستجو برای: kinetoplast dna
تعداد نتایج: 507472 فیلتر نتایج به سال:
The mitochondrial genome of Trypanosoma brucei is contained in a specialized structure termed the kinetoplast. Kinetoplast DNA (kDNA) is organized into a concatenated network of mini and maxicircles, positioned at the base of the flagellum, to which it is physically attached. Here we have used electron microscope cytochemistry to determine structural and functional domains involved in replicati...
Trypanosoma brucei, the causative agent of sleeping sickness (Human African Trypanosomiasis, HAT), contains a kinetoplast with the mitochondrial DNA (kDNA), comprising of >70% AT base pairs. This has prompted studies of drugs interacting with AT-rich DNA, such as the N-phenylbenzamide bis(2-aminoimidazoline) derivatives 1 [4-((4,5-dihydro-1H-imidazol-2-yl)amino)-N-(4-((4,5-dihydro-1H-imidazol-2...
Cesium chloride centrifugation of DNA extracted from cells of blood strain Trypanosoma lewisi revealed a main band, rho = 1.707, a light satellite, rho = 1.699, and a heavy satellite, rho = 1.721. Culture strain T. lewisi DNA comprised only a main band, rho = 1.711, and a light satellite, rho = 1.699. DNA isolated from DNase-treated kinetoplast fractions of both the blood and culture strains co...
Kinetoplast DNA (kDNA) is a novel form of mitochondrial DNA consisting of thousands of interlocked minicircles and 20-30 maxicircles. The minicircles replicate free of the kDNA network but nicks and gaps in the newly synthesized strands remain at the time of reattachment to the kDNA network. We show here that the steady-state population of replicated, network-associated minicircles only becomes...
For the last two decades, Dr. Majumder and his group have been involved in research on the enzyme DNA topoisomerases of Leishmania donovani, the causative agent for visceral leishmaniasis or kala-azar. He has made outstanding contribution towards the understanding of the biochemistry of the remarkable kinetoplast DNA (kDNA) network of the parasite and the role of DNA topoisomerases in kDNA meta...
BACKGROUND The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients. METHODOLOGY/PRINCIPAL FINDINGS We developed a new prototype of the OligoC-TesT based on kinetoplast DNA ...
Here, we show the morphological events associated with organelle segregation and their timing in the cell cycle of a reference strain of Leishmania (L.) amazonensis promastigotes, the main causative agent of Tegumentary leishmaniasis in the Americas. We show evidences that during the cell cycle, L. amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation, whic...
Kinetoplast DNA is the unusual mitochondrial DNA of trypanosomes. In Trypanosoma brucei it consists of about lo* minicircles (0.3 km) and lo* maxicircles (6 pm) catenated into a single network. The maxicircles are probably the equivalent of mitochondrial DNA in other organisms. Here we report that a fraction of the total cellular RNA from bloodstream form and culture form T. brucei hybridizes w...
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