The present study was conducted to investigate the existence of polymorphism at Kappa-casein (κ-CN) locus in the Nili-Ravi buffalo. A PCR-RFLP method was used to distinguish buffalo κ-CN alleles. The genotypes were confirmed through specific, clearly distinguishable DNA band pattern resulting from digestion with three selected restriction enzymes (HinfI, HaeIII and MaeII). Analysis of 163 anima...

Caseins are essentially concentrated in the colloidal fraction of ruminant milks as highly hydrated and mineralized spherical particles, termed casein micelles. They form a group of four peptide chains (alpha(s1), beta, alpha(s2) and kappa), encoded by four structural genes (CSN1S1, CSN2, CSN1S2 and CSN3, respectively) of which the expression is regulated by lactogenic hormones. These phosphopr...