a pancreatopeptidase (elastase) digest index was devised for a rapid and accurate estimation of protein quality. this index was calculated on the basis of all the amino acids released by an in-vitro elastase digestion, acid hydrolyses of same sample and the residue of enzyme hydrolyzed. the amino acids were determined by thin-layer chromatography. samples used were cooked white kidneybeans, coo...

pseudomonas aeruginosa ef2, atcc 9027 and atcc 19660 were grown in a continuous culture under tween 80 (polyoxyethylene sorbitan monooleate) limitation and optimum conditions (ph 6.5, 37°c at dilution rate of 0.05/h). culture supernatants were carefully removed and stored at -20°c. to purify the lipases, the culture supernatant was reduced in volume to approximately 10 ml by an ultrafiltration ...

BACKGROUND Total (i.e. free+sulfated) metanephrines in plasma is a biomarker for the diagnosis of pheochromocytoma/paraganglioma. Sulfated metanephrines must be completely deconjugated by perchloric acid hydrolysis or sulfatase treatment prior to analytical measurement to enable quantification by current techniques. In this report, we compare the yield and efficiency of both methods. METHODS ...

Liquid sugar is a sweetener that widely used in the food industry. Apart from starch, liquid can be produced cellulose hydrolysis by using cellulase enzymes. This study aims to obtain best concentration of enzymes production skin red dragon fruit. The uses method hydrolysing different concentrations (1%,2%,3%). results showed there was no significant influence on characteristics sugars resultin...

a thin-layer chromatography method has been developed and evaluated for the separation and determination of lysine and methonine in a mixture of amino acids obtained from acid or enzyme hydrolysis of protein. the solvent systems were (a) ethanol-ether, (b) phenol-water, (c) ethyl methyl ketone-pyridine-distilled water-glacial acetic acid. procedures in all the three stages were in the same dime...

The kinetics of chemical hydrolysis including neutral, acid- and base-catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent were studied at different temperatures. The rate constants and activation parameters were determined by following the build-up of fluorescence peak of the hydrolysis product 4-methylumbelliferone (4-MU). The time scale of esterase enzyme hydroly...

Mutants of Penicillium janthinellum NCIM 1171 were evaluated for cellulase production using both submerged fermentation (SmF) and solid state fermentation (SSF). Mutant EU2D-21 gave highest yields of cellulases in both SmF and SSF. Hydrolysis of Avicel and cellulose were compared using SmF and SSF derived enzyme preparations obtained from EU2D-21. Surprisingly, the use of SSF derived preparatio...

Mass spectrometry is a rapid, sensitive, and accurate quantitative approach for the direct monitoring of enzyme-catalyzed reactions that does not require a chromophore or radiolabeling and thus provides a viable alternative to existing analytical techniques. In this study the proteolysis of intact viral capsid proteins, the alpha-glucosidase-catalyzed hydrolysis of p-nitrophenyl-alpha-glucopyra...

Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments. Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ra...

The active-site aspartic acid residue, Asp-362, of Escherichia coli citrate synthase was changed by site-directed mutagenesis to Glu-362, Asn-362 or Gly-362. Only very low catalytic activity could be detected with the Asp----Asn and Asp----Gly mutations. The Asp----Glu mutation produced an enzyme that expressed about 0.8% of the overall catalytic rate, and the hydrolysis step in the reaction, m...