The fetal hemoglobin in the affected members of three Greek families with the hereditary persistence of fetal hemoglobin has only gamma-chains of the type with alanine in position 136. Although certain Negro families had been considered to have only this type of gamma-chains in their fetal hemoglobin, further studies required that they be reclassified. Consequently, the Greek cases are the sole...

Three widely used commercial hemoglobin electrophoresis kits were studied to ascertain specificity, accuracy, precision, and characteristics of product information. Two hundred and ninety-six hemoglobin samples were used to evaluate each kit. Except for poor separation of hemoglobin F by one kit, all three kits gave generally satisfactory results. We give specific recommendations for improving ...

T HE HUMAN HEMOGLOBINS consist of two pairs of peptide chains.1’2 Adult hemoglobin ( Hb-A ) has two chains designated a and two chains designated iB; it is thus represented as 2A $2A#{149}Fetal hemoglobin (Hb-F ) has two a chains, which are similar if not identical in structure to those of Hb-A, but a pair of chemically distinct chains, the y chains replacing the $ chains.3’4 Hb-F is thus repre...

By KARL SINGER, BASIL ANGELOPOULOS AND BRACHA RAMOT I T IS N(.)W well established that the erythrocytes of normal newborns contain about 50 to 90 per cent of fetal (type F) hemoglobin, the remainder being supplied by the normal adult (type A) pigment. As a rule, the fetal hemoglobin is rapidly replaced by the adult compound and after 7 to 12 months the embryonic pigment is no longer demonstrabl...

CLINICAL MANIFESTATIONS OF INHERITED ABNORMAL HEMOGLOBINS. I. THE / \. INTERACTION OF HEMOGLOBIN-S WITH HEMOGLOBIN-D. II. INTERACTION / OF HEMOGLOBIN-E AND THALASSEMIA TRAIT Phillip Sturgeon, Harvey A. Itano and William R. Bergren 389 STUDIES ON ABNORMAL HEMOGLOBINS. XI. SICKLE CELL-THALASSEMIA DISEASE IN THE NEGRO. THE SIGNIFICANCE OF THE S + A + F AND S -4A PATTERNS OBTAINED BY HEMOGLOBIN ANA...

We have analyzed the synthesis of fetal hemoglobin (HbF) in the differentiated erythroid colonies produced by erythropoietin-responsive committed erythroid progenitors in plasma cultures of human marrow and peripheral blood. Two independent biochemical techniques. carboxymethyl-cellulose column chromatography of 3H-leucine-Iabeled globin. and polyacrylamide gel electrophoresis of 59Fe-labeled h...

Specific antibodies to human fetal hemoglobin were prepared and, after conjugation with a fluorescent dye, were used to determine the distribution of Hb F-containing cells in blood smears from normal adults and individuals with hereditary and acquired conditions associated with abnormal levels of Hb F. The mean proportion of F-cells in normal persons was 2.7% +/- 1.4%, with a range of 0.5%-7.0%...

Members of 7 large families, containing 20 patients with sickle cell anemia (SS) characterized by high levels of fetal hemoglobin (HbF), were studied using immunofluorescence to count F cells and a radioimmunoassay to measure small amounts of HbF. In five of these families, one of the sickle cell trait (AS) parents had a much higher HbF and F-cell count than the other; in one family, both paren...

Recent interest in therapies for sickle cell anemia based on elevating fetal Hb has made accurate estimates of the sparing effect of fetal Hb (Hb F) and other non-sickle Hbs on sickle Hb (Hb S) polymerization essential. We have developed a technique, using HbCO as surrogate for HbO2, that enables us to assess the solubility of Hb S as a function of ligand saturation under conditions that mimic ...