INTRODUCTION The long-lasting persistence of hepatitis B virus (HBV) genomes in the liver (with or without detectable HBV DNA) of individuals with negative for HBV surface antigen (HBsAg) is termed occult HBV infection (OBI). The present study is a part of the follow up on efficacy of vaccination, 10 years post inception, and was designed to understand the prevalence of Occult Hepatitis B infec...

61 By using a novel real time genotyping and quantitative PCR (GQ-PCR) assay, we compared with 62 direct sequence analysis, reverse hybridization, and the multiplex PCR by detecting 127 HBV 63 infected patients. We found that GQ-PCR had the highest concordance with sequence analysis and 64 the highest detection rate for mixed genotype detecting. 65

BACKGROUND & AIMS Reported HBV drug resistance mutations among previously untreated patients with chronic hepatitis B are variable. Whether resistant HBV strains are transmitted in the acute setting is uncertain. We sought to document the presence of antiviral resistance (AVR) mutations in patients with acute HBV (AHB) infection. METHODS AHB infection was defined by HBsAg/IgM anti-HBc positiv...

Implication for health policy/practice/research/medical education: Molecular virology methods are developing rapidly and allow new insights into molecular epidemiology of viral infections. Despite the recent introduction of advanced methodology to genotype hepatitis B virus, using old but rapid and inexpensive genotyping tools like restriction fragment length poly-morphism have been utilized wi...

Occult hepatitis B virus (HBV) infections have been identified in patients with chronic hepatitis C virus (HCV) infection, although the clinical relevance of occult HBV infection remains controversial. We searched for serum HBV DNA in 106 HBsAg negative/anti-HBc positive patients with chronic HCV infection and in 150 blood donors HBsAg negative/anti-HBc positive/anti-HCV negative (control group...

conclusions in our study, only hbv genotype d was found among all the positive hbsag and occult hbv infection. moreover, high prevalence (40.7%) of occult hbv infection was determined among patients suffered from cryptogenic cirrhosis. results eleven (40.7%) out of 27 hbv cryptogenic cirrhosis and all 11 hbsag-positive patients were positive for hbv dna. the seroprevalences of hepatitis b virus...

OBJECTIVE To develop a convenient; sensitive; accurate; and economical technique for genotyping of hepatitis B viruses (HBVs). METHODS The mercapto-modified B1; B2; C1; and C2-specific genotyping probes consisted of two probes for each HBV genotype that served as a double verification system. These probes were fixed on the surface of No. 1; 2; 3; and 4 gold electrodes; respectively; via Au-S ...

OBJECTIVES To analyze the role of HLA genotypes in persistence of chronic hepatitis B in Western India. METHODS HLA genotyping for class II-DR was done in 26 subjects having chronic hepatitis B infection (HBsAg positive) and in 100 healthy controls. Statistics were done using Halden's modification of Woolf's formula. RESULT Significant association of chronic hepatitis B infection was found ...

BACKGROUND Hepatitis B virus (HBV) infection significantly contributes to the onset of liver disease and hepatocellular carcinoma. Understanding the pathogenesis of HBV infection susceptibility could help us to control HBV infection effectively. OBJECTIVES This study investigated single nucleotide polymorphisms (SNPs) of the tripartite motif-containing 22 (TRIM22) gene associated with HBV inf...

DNA methyltransferase (DNMT) 1 is the key enzyme responsible for DNA methylation, which often occurs in CpG islands located near the regulatory regions of genes and affects transcription of specific genes. In this study, we examined the possible association of DNMT1 polymorphisms with HBV clearance and the risk of hepatocellular carcinoma (HCC). Seven common polymorphic sites were selected by c...