Using an automated immunoprecipitin method, we assayed human sera for 10 proteins: haptoglobin, orosomucoid, transferrin, alpha1 antitrypsin, alpha2-macroglobulin, IgG, IGa, IgM, complement C3, and complement C4. Blood from 14 healthy subjects (25-40y) was sampled on six separate days. From each venipuncture serum was divided into four eliquots; two were assayed on the day of venipuncture and t...

Haptoglobin is one of acute phase glycoproteins often used as markers in glycopathology studies. In this work the oligosaccharide structures of haptoglobin from 'healthy' subjects have been studied in detail, taking into consideration the possible dependence of glycosylation on the phenotype. About 75% of charged haptoglobin glycans were of biantennary complex structure, and some of them lacked...

Gel filtration of serum by use of dextran bisacrylamide beads ("Sephacryl S-200," fractionation range: 5000-250 000 daltons), separates the serum proteins into three distinct peaks. We used an eluent containing (per liter) 140 mmol of sodium, 1.10 mmol of calcium, 0.50 mmol of magnesium, and 10 mmol of 2-([2-hydroxy-1, 1-bis(hydroxymethyl)ethyl]amino)ethanesulfonate buffer (pH 7.43 at 37 degree...

Nineteen patients with morphological abnormalities of the red blood cells are described, and these formed approximately 3% of the total cases of cardiac valvular disease. In two patients the abnormal blood film developed after the insertion of an aortic and mitral valve prosthesis respectively, but in another two patients the abnormal blood film was corrected by aortic valve surgery. Anisopoiki...

Serum concentrations of fucose, sialic acid, and eight acute phase proteins were measured in single specimens from patients with cancer in order to determine whether the raised concentrations of protein bound sugars commonly found in cancer correlate with increased concentrations of the acute phase proteins. Strong positive correlations were found only with alpha 1-acid glycoprotein, alpha 1-an...

consumption in experimental and clinical acute pulmonary thromboembolism. Crit Care Med 2013; 41: e118–e124. 6 Levi M. Disseminated intravascular coagulation or extended intravascular coagulation in massive pulmonary embolism. J Thromb Haemost 2010; 8: 1475–1476. 7 Leitner JM, Jilma B, Spiel AO, et al. Massive pulmonary embolism leading to cardiac arrest is associated with consumptive coagulopa...

Haptoglobin types were determined in 626 individuals living in the State of São Paulo (Brazil). Of these, 484 had Hb AA, 31 major beta thalassemia, 43 minor beta thalassemia, 14 Hb SS, and 54 Hb AS. Frequency distribution of the three most common types observed among patients with type beta thalassemia differed significantly from that observed in the Caucasian group with Hb AA. There was a sign...

The incidence and severity of chronic intravascular hemolysis was evaluated in a total of 41 patients following mitral valve replacement. Valve replacement was with a gamma-radiated frame-mounted aortic homograft in 21 patients and with a composite seat Starr-Edwards prosthesis, model 6310 or 6320, in 20 patients. The parameters used to assess hemolysis were hemoglobin, hematocrit, reticulocyte...

A 55-year old italian, male, was referred to our hospital for the evaluation of fever of unknown origin (F.U.O.) associated with jaundice and a severe multiorgan failure. This expatriate worker had returned from Nigeria three days earlier with some prodromal symptoms: malaise, headache myalgia, anorexia; he developed a very high temperature at once ranging between 40 to 41°C (105 to 106°F) asso...

Natural resistance of humans to the cattle pathogen Trypanosoma brucei brucei has been attributed to the presence in human serum of nonimmune factors that lyse the parasite. Normal human serum contains two trypanosome lytic factors (TLFs). TLF1 is a 500-kDa lipoprotein, which is reported to contain apolipoprotein A-I (apoA-I), haptoglobin-related protein (Hpr), hemoglobin, paraoxonase, and apoA...