Protocol: Reactions were performed as described (2) using 1 ng of DNA and 100 pmoles of each primer. DNA was amplified for 20 cycles using a Perkin Elmer Cetus DNA Thermal Cycler: denaturation, 94°C, 1 min; annealing, 60°C, 1 min; and extension, 70°C, 1.5 min. Ten /il of the reaction was analyzed directly on a 2.5 % NuSieve GTG agarose gel run in Tns-borate buffer. The amplified DNA fragments v...

A fast-response and wide-view fringe-field switching LCD using patterned common electrodes is proposed. By applying a restoring pulse voltage on common electrodes, LC decay process is expedited. The GTG decay time can be reduced by >7X, depending on the applied erasing voltage. This new mode also preserves the wide-view characteristics as the conventional FFS mode. It is attractive for reducing...

We discuss the applications of Gauge Theory Gravity (GTG) within the language of geometric algebra to the Dirac equation in the de Sitter and Rindler metrics as well as to the Unruh effect. We rederive the Hawking temperature for each case. The derivation of both the correct Fermi-Dirac statistics as well as the Hawking temperature may suggest that the method of calculations we employ here geom...

Meningiomas are classified as benign, atypical, or anaplastic. The majority are sporadic, solitary, and benign tumors with favorable prognoses. However, the prognosis for patients with anaplastic meningiomas remains less favorable. High resolution genomic profiling has the capacity to provide more detailed information. Therefore, we analyzed genomic aberrations of benign and atypical meningioma...

Three hundred and eight presumed enterococcal isolates were recovered from Bryndza, a soft sheep milk cheese. The cheese samples were obtained from five different commercial distributors in Slovakia and were taken at three different seasonal intervals. All isolates were identified to the species level using genotypic tools. Species-specific PCR using ddl genes highlighted the predominance of En...

We studied a 26-year-old Portuguese patient with recessively transmitted hereditary hemolytic anemia. Protein 4.2 was absent from red cell ghosts by Western blotting. Although the 4.2 mRNA was not detected in Northern blots, it was shown to be present by a procedure based on nested reverse transcription-polymerase chain reaction (RT-PCR). Partial nucleotide sequencing disclosed a one-nucleotide...

BACKGROUND Tuberculosis continues to be one of the leading causes of death worldwide and in the American region. Although multidrug-resistant tuberculosis (MDR-TB) remains a threat to TB control in Panama, few studies have focused in typing MDR-TB strains. The aim of our study was to characterize MDR Mycobacterium tuberculosis clinical isolates using PCR-based genetic markers. METHODS From 20...

autism is a complex neuropsychiatric disorder that manifests in early childhood. although the etiology is unknown yet but, new hypothesis focused on identifying the key genes related to autism may elucidate its etiology. the main objective of the present study was to verify the value of karyotyping in autistic children and identifying association between chromosome abnormalities and autism.we e...

Oligonucleotide probes specific for simple tandem repeat sequences produce individual specific DNA fingerprints in man and all animal species so far. In the present investigation (r)-p labeled oligonucleotide probes (GTG)5, (GT)8 and (GGAT)4 were used as multilocus probes to detect hypervariable microsatellites in the Indian dromedary camel population. Oligo probes were radiolabelled and hybrid...