نتایج جستجو برای: glass capillary micropipette
تعداد نتایج: 110213 فیلتر نتایج به سال:
We characterize a new geometry for single-molecule detection with flow for use with a submilliliter drop of sample on an inverted confocal microscope. The solution is sucked into a glass capillary positioned above the ellipsoidal confocal volume so that molecules traverse the longest axis of the ellipsoid for greatest photon yield. Decreased spacing between the capillary tip and laser focus giv...
The red cell's spectrin-actin network is known to sustain local states of shear, dilation, and condensation, and yet the short actin filaments are found to maintain membrane-tangent and near-random azimuthal orientations. When calibrated with polarization results for single actin filaments, imaging of micropipette-deformed red cell ghosts has allowed an assessment of actin orientations and poss...
We have developed an 8-ch capillary-based dispensing workstation with a variable capillary pitch mechanism. The capillary intervals can be varied from 1 to 9 mm to dispense different solutions simultaneously at an arbitrary dispensing pitch, allowing direct dispensing from microplates to integrated analytical systems. To evaluate the precision of its dispensing performance, droplets of Rhodamin...
In this chapter, we describe the fabrication and use of microfluidic capillary systems (CSs) made in soft, transparent polydimethylsiloxane (PDMS). Sixteen microfluidic CSs, each containing a loading pad, a microchannel, and a capillary pump are engraved in a single PDMS chip. The CSs are used for two applications, firstly to pattern fibronectin on glass surfaces to locally control the adhesion...
Use of high-resolution open tubular glass capillary columns to separate acidic metabolites in urine.
We used high-resolution glass capillary columns to study the trimethylsilyl derivatives of some acidic metabolites found in pooled urine specimens form control and postpartum subjects. About 30 compounds were identified by gas chromatography-mass spectrometry-computer techniques. In general, open tubular capillary columns effect better resolution of trimethylsilyl derivatives of organic acids t...
An extremely rapid on-chip separation of DNA from its single-base substituted mutant is described. By using affinity capillary electrophoresis (ACE) in a poly(dimethylsiloxane) (PDMS)-glass hybrid microdevice, we achieved the separation within 5 s.
Self-assembly of colloidal spheres confined within cells of different shapes formed with two slides under capillary forces are studied. It is found that by controlling the shape of the cell the curvature of the drying front can result in a significant effect on the self-organization process. A curved drying front formed within parallel slides is always associated with growth of colloidal crysta...
Gallium ultramicroelectrodes for amperometric measurements in scanning electrochemical microscopy were fabricated by introduction of liquid Ga into drawn glass micropipets. Cyclic voltammetry of Ru(NH(3))(6)(3+) and the use of this species for SECM imaging is described. Double-barrel micropipet tips with a Ga amperometric electrode and an ion-selective (K(+)) potentiometric probe can also be co...
Aflatoxin G2 was used to test the speed and accuracy of the microapplicator and the micropipette. The 50% lethal dose of both assay systems was approximately the same, and 4.0 mug of G2 had an 85% lethal effect in both systems. The 50% lethal dose of the microapplicator and the micropipette was lower than that of the syringe but, of these, only the micropipette can combine the accuracy of the m...
This paper describes a glass-capillary-accessible dynamic microarray for an efficient microinjection of zebrafish embryos. The microfluidic channel has an array of holes at the trapping sites and enabled a rapid alignment of eggs, direct injection, observation, and selective retrieval of embryos. Fertilized zebrafish eggs can be loaded without pumping, and genes of interest or antisence oligos ...
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