With the increasing interest in the diagnosis of malignant cells in various exudates and body fluids, a method demonstrating certain nucleolar changes in considerable detail may be of value. I t is t he purpose of this paper to present such a method and to discuss briefly the nucleolar changes in benign and malignant tissues and also to point out the potential applicability of this method as an...

AIMS To verify whether the proposed new silver staining method compares favourably with other well established methods in the detection of Helicobacter pylori in gastric biopsies. METHODS One hundred and forty pairs of antral and fundic biopsies, routinely formalin fixed and paraffin wax embedded, from 70 consecutive unselected patients were stained with haematoxylin and eosin, modified Giems...

The karyological features of Akis subtricostata was determined for the first time with conventional and silver nitrate staining. diploid number 2n=16 meioformula 7+neoXY represents a deviation from modal karyotype Coleoptera. pericentromeric heterochromatin detected both Giemsa In addition to determining single possible NOR on prophase I nuclei, AgNO3 revealed that several telomeric regions mit...

Helicobacter pylori is thought to represent a significant etiopathogenic factor in diseases of the upper gastrointestinal tract. It seems, therefore, important to elaborate effective techniques for its detection. The aim of the present study was to evaluate the effectiveness of Helicobacter pylori detection using the PCR technique on paraffin sections with various pairs of primers and to compar...

Six diagnostic methods (Giemsa staining, Ziehl-Neelsen staining, auramine-rhodamine staining, Sheather's sugar flotation, an indirect immunofluorescence procedure, and a modified concentration-sugar flotation method) for the detection of Cryptosporidium spp. in stool specimens were compared on the following attributes: diagnostic yield, cost to perform each test, ease of handling, and ability t...

Several tick-borne diseases with different etiological agents such as rickettsia, protozoa, bacteria and viruses can infect domestic ruminants. The only common feature between these diseases is that they can all be transmitted by ticks. Of these, Anaplasma spp. can cause diseases in the livestock with high economical losses. The Giemsa staining of blood smear is the common traditional method fo...

The aim of this study was to investigate diagnostic methods for cryptococcal meningitis (CM). A retrospective analysis was conducted for 31 patients with CM confirmed by etiologic detection of cerebrospinal fluid in our hospital in the past 5 years. Nineteen cases in 31 patients were confirmed with CM in the first diagnosis, with a misdiagnosis rate of 38.7%. The positive rates of cryptococcus ...

BACKGROUND This study aimed to define the occurrence of different organisms causing vulvovaginitis; to evaluate different laboratory methods used for diagnosis of Trichomonas vaginalis (T. vaginalis); and to evaluate the direct score system and clue cell method compared with culture for diagnosis of bacterial and T. vaginalis vaginosis. METHODOLOGY Clinical and laboratory evaluations were per...

Malaria is a responsible for approximately 600 thousand deaths worldwide every year. Appropriate and timely treatment of malaria can prevent deaths but is dependent on accurate and rapid diagnosis of the infection. Currently, microscopic examination of the Giemsa stained blood smears is the method of choice for diagnosing malaria. Although it has limited sensitivity and specificity in field con...