BACKGROUND/AIMS The use of "fresh" (hypothermically stored) and frozen amniotic membrane (AM) was compared in a patient with cicatricial pemphigoid with stem cell failure. The viability of both "fresh" and frozen AM epithelial cells was assessed after storage. METHODS AM was stored at either +4 degrees C ("fresh") or at -80 degrees C (frozen). A "fresh" graft was applied to the cornea followi...

BACKGROUND Intraoperative pathologic diagnosis of bone and soft tissue lesions is an important yet challenging tool in clinical musculoskeletal oncology practice. There is limited information in the literature addressing the practical issues commonly encountered regarding intraoperative frozen section of musculoskeletal lesions. METHODS A literature review and retrospective review of practica...

The recent rise in speed and efficiency of new sequencing technologies have facilitated high-throughput sequencing, assembly and analyses of genomes, advancing ongoing efforts to analyze genetic sequences across major vertebrate groups. Standardized procedures in acquiring high quality DNA and RNA and establishing cell lines from target species will facilitate these initiatives. We provide a le...

Testicular tissue cryopreservation is the only potential option for fertility preservation in pre-pubertal boys exposed to gonadotoxic treatment. Completion of spermatogenesis after in vitro maturation is one of the future uses of harvested testicular tissue. The purpose of the current study was to evaluate the effects of vitamin A on in vitro maturation of fresh and frozen-thawed mouse pre-pub...

The elasticity of biological tissues is one the physical characteristics and has attracted attention as a clinical diagnostic parameter. can be determined on microscopic scale with speed sound (SoS) measurements using acoustic microscopy. In SoS measurements, thin-sliced section attached to glass slide in same manner light specimen. There are two main methods for preparing thin sections: paraff...

Optical properties obtained from spectrophotometer measurements of reflectance and transmittance were determined for both frozen-ground and intact soft tissues. The tissues used in these experiments were calf aorta, rat jejunum, and rabbit sciatic nerve. Tissue specimens from each tissue type were frozen in liquid nitrogen and then ground with a pestle and mortar into a fine powder. A tissue pa...

A tissue biopsy is usually a critical aspect in guiding appropriate initial management in patients with musculoskeletal tumours. We have previously outlined the role of intra-operative frozen section in both the determination of adequacy of a biopsy and for its diagnostic utility. In this article, the options and techniques for intra-operative pathological evaluation, namely frozen section, fin...

BACKGROUND Cryoelectrolysis is a new minimally invasive tissue ablation surgical technique that combines the ablation techniques of electrolytic ablation with cryosurgery. The goal of this study is to examine the hypothesis that electrolysis can take place in a frozen aqueous saline solution. METHOD To examine the hypothesis we performed a cryoelectrolytic ablation protocol in which electroly...

Catalase (CAT, EC 1.11.1.6) activity was measured in flesh tissue of six apple cultivars (Malus domestica Borkh. cvs. Braeburn, Gala, Jonagold, McIntosh, Red Delicious, and Spartan). Activity of CAT was determined for fresh and frozen tissue of the same fruit. Freezing resulted in reductions of 50 to 90% in CAT activity compared with the activity measured in crude extracts from fresh tissues. T...

colorectal cancer is the most common gastrointestinal cancer and the second leading cause of cancer deaths worldwide. the purpose of this study is to investigate the prevalence of epstein-barr virus in patients with colorectal carcinomas and polyps in comparison with healthy people. in this analytical  case-control study, 15 patients with colorectal cancer and 20 patients with colorectal polyp ...