The MTT/MTS in vitro cell proliferation assay is one of the most widely used assays for evaluating preliminary anticancer activity of both synthetic derivatives and natural products and natural product extracts. The highly reliable, colorimetric based assay is readily performed on a wide range of cell lines. This assay gives an indication of whole cell cytotoxicity; however, to determine the ex...

DNA methyltransferases (MTases), a family of enzymes that catalyse the methylation of DNA, have a profound effect on gene regulation. A large body of evidence has indicated that DNA MTase is potentially a predictive biomarker closely associated with genetic disorders and genetic diseases like cancer. Given the attention bestowed onto DNA MTases in molecular biology and medicine, highly sensitiv...

Advances in detection technologies have enabled an increased use of cell-based functional assays in early drug discovery, in particular for G protein-coupled receptors. Screening assays that use live cells are less prone to generate false positives than assays using lysed cell samples. The use of cryopreserved cells instead of cells that are continuously maintained in culture decreases day-to-d...

Apple is one of the most important fruits in world. The elaboration apple-based products such as cider or apple juice generates around 3–4.2 million tonnes by-product that are annually discarded. has been described an interesting source bioactive compounds, including polyphenols. These valuable components, once extracted, could enable usage a natural antioxidant ingredient. aim present work was...

background and aims: celery mosaic virus (cemv) is one of the causal agents of viral diseases in celery (apium graveolens). cemv is a member of the potyvirus genus in potyviridae family. the virus is naturally transmitted by aphids in a non-persistent manner. during growing season 2006-2007 viral disease symptoms were observed in celery fields grown in tehran province (bage daneshkade, mohamads...

Enzyme activities have been used as indicators of soil quality and changes in biogeochemical function due to management or perturbations. The objective of this study was to answer a number of methodological questions regarding sampling schemes, sample handling recommendations, and assay procedures to facilitate the use of enzyme assays in the tropical highlands of East Africa. We used paranitro...

Purified tubulin or microtubules are widely used for in vitro assays related to their function, such as activities of modifying enzymes and motor protein activity. This paper gave a review purification (MTs) using two different methods. One method MTs depended on polymerization depolymerization. were polymerized at 37 °C with taxol the presence. Another is purify by affinity column chromatograp...

Wood of coniferous trees (softwood), is a globally significant carbon sink and an important source biomass. Despite that, little known about the genetic basis softwood cell wall biosynthesis. Branching xylan, one main hemicelluloses in secondary walls, with glucuronic acid (GlcA) critical for biomass recalcitrance. Here, we investigate decoration patterns xylan by conifer GlucUronic substitutio...