The distribution and metabolism of [(14)C]aflatoxin B(1) in chicken tissues were further investigated. Previously dried and frozen ethyl acetate extracts of liver, heart, gizzard, breast, leg, blood, and fecal samples were obtained from either layer or broiler chickens fed subclinical levels of [(14)C]aflatoxin B(1). Treatment of these extracts with either carboxypeptidase A, leucine aminopepti...

Since the discovery of enzymes as biological catalysts, study of their enormous catalytic power and exquisite specificity has been central to biochemistry. Nevertheless, there is no universally accepted comprehensive description. Rather, numerous proposals have been presented over the past half century. The difficulty in developing a comprehensive description for the catalytic power of enzymes ...

Enzymes are biological catalysts (also known as biocatalysts) that speed up biochemical reactions in living organisms, and which can be extracted from cells and then used to catalyse a wide range of commercially important processes. This chapter covers the basic principles of enzymology, such as classification, structure, kinetics and inhibition, and also provides an overview of industrial appl...

The present study mainly focused on quantification and comparison of non enzymatic antioxidants from pulp extracts of nine varieties of banana viz Kadali, Karpooravalli, Monthan, Nenthiran, Pachainadan, Poovan, Rasthali, Robusta and Sevvazhai. The non enzymatic antioxidants such as total reduced glutathione, vitamin A, vitamin C, vitamin E were quantified from banana pulp extract. The data obta...

Background: The enzyme tyrosinase is a copper-containing polyphenol oxidase, which is involved in melanogenesis. It catalyzes the conversion of tyrosine to melanin pigments in mammals. Tyrosinase plays an important role in enzymatic browning, which causes discoloration and loss economic value of many plant food products. This enzyme is widely distributed in fungi, higher plants and animals. Ob...

The product of the first two reactions in the enzymatic degradation of myo-inositol by extracts of Aerobacter aerogORBS, D-2,3-diketo-4-deoxy-epi-inositol, serves as the substrate for another enzyme also present in these extracts. The enzyme was partly purified and could be shown to catalyze, presumably after a preliminary isomerization, the hydrolytic cleavage of the inositol ring. The product...

The main aim of this work was to investigate the effects of temperature and nitrogen concentration on the antioxidant potential of extracts from Spirulina (Arthospira) platensis biomass. S. platensis biomass obtained at 35oC and with 1.875 g.L or 2.5 g.L of sodium nitrate in the culture medium presented higher concentrations of phenolic compounds. The antioxidant potential of methanol extracts ...