Ochratoxin A (OTA) is a toxicant commonly present in many food products. Conventionally, immuno-affinity analysis is applied to rapidly screen the presence of OTA in food. However, antibodies are expensive. In this study, we present a new approach for selectively enriching OTA from aqueous samples using human serum albumin (HSA) bound magnetic beads as the affinity probes, followed by the analy...

Tc is a radionuclide widely used for imaging diagnosis in nuclear medicine. In Brazil it is obtained by elution from 99 Mo-Tc generators supplied by the Nuclear Energy Research Institute (IPEN). The elution is carried out in radiopharmacy laboratories located in hospitals and clinics. Depending of the quality of the generator and conditions of use during the elution process, Mo can be extracted...

The LH receptor was solubilized from porcine testis homogenates by different detergents. Non-ionic detergents appeared to be the best ones regarding solubilization yield and recovery of active receptor after elution from hCG affinity gels. However, the final yield was not greater than 1-2% of the starting receptor activity. We also investigated the effect of added phospholipids (75% phosphatidy...

Each chick and human red cell contains approximately 300 sites capable of attaching influenza virus particles. These correspond to an area representing approximately 2 per cent of the red cell surface. Although the rate of attachment of PR8 to red cells is not diffusion-limited, when calculated on the basis of the total cell area, it does approach the theoretical maximum for interaction between...

buffer (50 mmol/liter Tris-HC1, pH 7.5, containing 300 mmol of NaCl per liter). The BB values are significantly different in samples 1, 3, and 8, where the true MB activity is >40 U/liter. The remaining samples with <40 U of MB per liter do not show any significant carryover of MB to BB. We are in the process of changing the MB buffer-salt content; this will essentially eliminate the MB to BB c...

DNA-protein cross-links are induced in mammalian cells by X-rays, ultraviolet light, fluorescent light, and numerous chemical carcinogens. Others have shown that these cross-links are repaired by normal cells but that excision repair-deficient xeroderma pigmentosum (XP) Group A cells, XP12BE, are deficient in repair of these bulky adducts. This paper compares the DNA-protein cross-link repair c...

Antibiotic-loaded bone cement (ALBC) is widely used in orthopaedic surgery for both prevention and treatment of infection. Little is known about the effect of different brand combinations of antibiotic and bone cement on the elution profile and mechanical strength of ALBC. Standardized specimens that consisted of one of the 4 brands of bone cement and one of the 3 brands of vancomycin were fash...

The antimicrobial properties and the elution characteristics of gentamicin-vancomycin-loaded hip spacers were studied in vivo and in vitro. Vancomycin elution was greater than gentamicin elution. The antibiotic concentrations in vivo were less than those in vitro. Not dependent on implantation duration, growth inhibition by spacers in vitro was observed for 2 weeks. The reason for protracted wo...