background and objectives: timely diagnosis of leptospirosis is essential for an effective treatment. large diversity of clinical symptoms has led leptospirosis diagnosis difficult. researchers have conducted many tests with wide-range of sensitivity and specificity to achieve novel diagnostic procedures which have higher sensitivity and specificity compared with previous tests and which are mo...

Four polyethylene glycol-mediated cell fusions yielded a total of 23 monoclonal antibodies (McAbs) specific for African horsesickness virus (AHSV). Two recognised the major core structural polypeptide, VP7, while one each was specific for the outer capsid proteins, VP2 and VP5. The remainder co-precipitated both VP2 and VP7. An inhibition ELISA and radio-immunoprecipitation revealed two types o...

An easy and rapid ELISA system, Filtration ELISA, to detect antibodies against bacterial cell surface antigens was developed using a 96-well filtration plate fitted with a 0.22microm membrane (MultiScreen-GV, Millipore). Bacterial whole cells were used as antigens without fixing the cells with formalin etc. The whole cell antigens were washed by vacuum filtration through a filter and resuspende...

background: the assays currently available for the detection of specific anti- toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack of a purified standardized antigen. the aim of this study was evaluation the recombinant toxoplasma gondii sag1 antigen for the serodiagnosis of acute and chronic toxoplasmosis. methods : this study describes an elisa u...

Synthetic peptide antigens were prepared for use in enzyme-linked immunosorbent assays (ELISAs) to detect serum antibodies against abortigenic strains of Chlamydia psittaci in livestock. Peptide antigens were identified with C. psittaci B577-immune sera by solid-phase scanning of overlapping octapeptides of variable domains (VDs) of the major outer membrane protein of C. psittaci serovar 1 (omp...

A modification of enzyme-linked immunosorbent assay (ELISA) was developed for the serological characterisation and identification of strains of Ureaplasma urealyticum. The eight recognised human serotypes of U urealyticum and antisera produced against them were used as reference for the evaluation and standardisation of the method. The serological profile illustrating reactions of antigen with ...