In order to fill in a decade-long information gap regarding the biological, serological and molecular data for plum tree viruses Bosnia Herzegovina, three-phase study combining symptom evaluation, assays with high-throughput sequencing (HTS) technology was conducted. The most frequently observed symptoms were discolorations form of ring patterns, bands irregular shapes, as well vein banding. Sh...

Restricted antibody reactivity to hepatitis C virus (HCV) synthetic peptides has been observed in HCV-infected patients on haemodialysis (HD). The aim of this study was to evaluate third-generation anti-HCV enzyme-linked immunosorbent assay (ELISA) test systems containing either synthetic peptide HCV antigens or recombinant HCV antigens or a combination of synthetic and recombinant antigens in ...

there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

Orchid plants are the members of Orchidaceae consisting of more than 25,000 species, which are distributed almost all over the world but more abundantly in the tropics. There are 177 genera, 1,125 species of orchids that originated in Thailand. Orchid plant collected from different nurseries showing Chlorotic and mosaic symptoms were observed on Vanda plants and it was suspected to infect with ...

There is an error in the tenth sentence of the Abstract. The correct sentence is: In addition, enzyme-linked immunosorbent assays were conducted to determine recent viral infections and 12 (1.3%) subjects were found to have been recently infected by the virus. There is an error in the first sentence of the “Recent infection to Japanese encephalitis virus” section of the Results. The correct sen...

OBJECTIVE To evaluate two commercially available ELISA-based kits against RT-PCR for the diagnosis of dengue virus infection in a Tertiary Care center in Karachi. METHODS During the 2006 Dengue outbreak, sera were collected from patients clinically classified as dengue fever and graded according to WHO grading. Out of these, 83 samples were selected randomly and analyzed using two different c...