In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...

5-Hydroxymethylcytosine (5hmC) is a modified base present at low levels in various mammalian cells, and it plays essential roles gene expression, DNA demethylation, genomic reprogramming. Herein, we develop label-free template-free chemiluminescent biosensor for sensitive detection of 5hmC DNAs based on 5hmC-specific glucosylation, periodate (IO4+) oxidation, biotinylation, terminal deoxynucleo...

  Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...

Purpose. To substantiate the definition of technics as attributive characteristics a human being and necessity its orientation towards flourishing in context new anthropological models 21st century. Theoretical basis. Correlation between technics, technology essence is examined. The role traced at different historical stages development. Negative positive effects digital development upon contem...