We present an initial end-to-end performance evaluation of Intel’s R 10-Gigabit Ethernet (10GbE) network interface card (or adapter). With appropriate optimizations to the configurations of Linux, TCP, and the 10GbE adapter, we achieve over 4-Gb/s throughput and 21s end-to-end latency between applications in a local-area network despite using less capable, lower-end PCs. These results indicate ...

Pseudomonas oleovorans NCIMB 6576 and Ralstonia eutropha 10442 were used for the production of Polyhydroxyalkanoates (PHA) from industrial waste cooking oils, bacteria cultured on tryptone soya broth (TSB) Tryptone agar (TSA). The growth pattern bacteria, serial dilution viable counting was done using Miles Misra method, 0.5ml (500 µl) sample transferred aseptically into test tubes filled with ...

Digital polymerase chain reaction (PCR) is an emerging absolute quantification method based on the limiting dilution principle and end-point PCR. This methodology provides high flexibility in assay design without influencing quantitative accuracy. This article describes an assay to quantify HIV DNA that targets a highly conserved region of the HIV-1 genome that hampers optimal probe design. To ...

A new rapid method for titrating baculovirus stocks has been developed using a novel cell line Sf-9 Easy Titer (Sf-9ET). The Sf-9ET cell line has been transfected with plasmid DNA containing the enhanced green fluorescent protein (eGFP) gene under the control of the baculovirus polyhedrin promoter. When used in the titration assay, the Sf-9ET cells turn green when they are infected with baculov...

Pathways and dilution of a point source ocean discharge in the farfield (approximately to 10-66 km) were measured using the deliberate tracer sulfur hexafluoride (SF6). The injection of SF6 was performed by bubbling the gas over a period of 6 days into an ocean outfall pipe discharging into the southeast Florida coastal ocean. The surface SF6 concentrations show that the discharged water flowed...

INTRODUCTION End expiratory lung volume (EELV) measurement in the clinical setting is routinely performed using the helium dilution technique. A ventilator that implements a simplified version of the nitrogen washout/washin technique is now available. We compared the EELV measured by spiral computed tomography (CT) taken as gold standard with the lung volume measured with the modified nitrogen ...

The bioethanol content of two samples of biofuels was determined directly, after simple dilution in decane, by means of an amperometric catalase enzyme biosensor working in the organic phase, based on substrate antagonisms format. The results were good from the point of view of accuracy, and satisfactory for what concerns the recovery test by the standard addition method. Limit of detection (LO...

This article examines security within the context of an architecture based on quality. To achieve quality, the elements of continuity, confidentiality, and integrity need to be provided. Confidentiality as it relates to quality can be defined as access control. It includes an authorization process, authentication of users, a management capability, and auditability. This last element, auditabili...

Bioassay by end-point dilution has been used for decades for routine determination of prion infectivity titre. Here we show that the new protein misfolding cyclic amplification with beads (PMCAb) technique can be used to estimate titres of the infection-specific forms of the prion protein with a higher level of precision and in 3-6 days as opposed to 2 years, when compared with the bioassay. Fo...

Although reproducible results can be obtained in photometric set-point titrations of submicromolar amounts (5 -lo+ M and higher) of calcium(I1) and zinc(I1) by using an ? automatic titrator, the systematic error can be considerable. This error is partly intrinsic ; in nature, but there is an additional error caused by dilution in successive titrations_ Accurate values can be obtained only when ...