نتایج جستجو برای: denaturing

تعداد نتایج: 4945  

2013
P. E. Dickerson A. R. Trim John Innes

The four components of the genome of cowpea chlorotic mottle virus have been prepared in a highly active and highly purified state by a method based on their resolution by polyacrylamide gel electrophoresis. Activity and purity have been confirmed by gel electrophoresis under denaturing and non-denaturing conditions, infectivity tests on whole plants, translation in an mRNA dependent rabbit ret...

2016
Jessica Lleixà Maria Manzano Albert Mas María del C. Portillo

The inoculation of wines with autochthonous yeast allows obtaining complex wines with a peculiar microbial footprint characteristic from a wine region. Mixed inoculation of non-Saccharomyces yeasts and S. cerevisiae is of interest for the wine industry for technological and sensory reasons. However, the interactions between these yeasts are not well understood, especially those regarding the av...

2015
Jie Liu Jianan Wang Guanghai Gao Mark G. Bartlam Yingying Wang

Fungal communities perform essential functions in biogeochemical cycles. However, knowledge of fungal community structural changes in river ecosystems is still very limited. In the present study, we combined culture-dependent and culture-independent methods to investigate fungal distribution and diversity in sediment on a regional scale in the Songhua River catchment, located in North-East Asia...

Gao, M.L., Hou, H.M., Liu, Y., Sun, L.M., Zhang, G.L.,

The intestinal bacterial diversity of Stichopus japonicus was investigated using 16S ribosomal RNA gene (rDNA) clone library and Polymerase Chain Reaction/Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (OTUs) with sequence similarity ranging from 88 to 100%. The cov...

Journal: :The Journal of General Physiology 2003
L. A. Brown W. J. Crozier

In spite of obvious possible sources of disturbance, the "velocity of killing" of organisms at supranormal temperatures, properly determined, tends to adhere to the Arrhenius equation for relation to temperature. Over certain ranges of temperature the relationship between log velocity of killing and 1/T degrees abs. is linear. Interpreted as due to the thermal denaturing of protein, it is possi...

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