Rhodococcus equi ATCC13557 was selected as a model organism to study oestrogen degradation based on its previous ability degrade 17α-ethinylestradiol (EE2). Biodegradation experiments revealed that R. unable metabolise EE2. However, it able E2 with the major metabolite being E1 no further of E1. conversion into incomplete, 11.2 and 50.6% degraded in mixed (E1-E2-EE2) E2-only conditions, respect...

2. Ravindranath V, Anandatheerthavarada HK and Shankar SK, NADPH cytochrome P-450 reductase in rat, mouse and human brain. Biochem Pharmaco139: 1013-1018, 1990. 3. Ravindranath V, Anandatheerthavarada HK and Shankar SK, Xenobiotic metabolism in human brain-presence and cytochrome P-450 and associated monooxygenases. Brain Res 496: 331-335, 1989. 4. Anandatheerthavarada HK, Shankar SK and Ravind...

Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is...

The in vitro metabolism of fenthion and its sulfoxide (fenthion sulfoxide) in sea bream (Pagrus major) and goldfish (Carassius auratus) was investigated and compared with that in rats. Fenthion was oxidized to fenthion sulfoxide and the oxon derivative, but not to its sulfone, in the presence of NADPH by liver microsomes of sea bream, goldfish, and rats. These liver microsomal activities of the...

A “cocktail”of several probe drugs is often used to evaluate metabolic activity of multiple cytochrome P450 enzymes in one session. Some interactions among probe drugs can appear and may impact the rate of biotransformation of other ones. Our presented work was to aim on the influence of bupropion on rat cytochrome P450-mediated metabolism of tolbutamide. The biotransformation rates of tolbutam...

The relative increase of the 7-ethoxycoumarin-O-deethylase and the 6,7-dimethoxycoumarin-O-demethylase activities were found 93 and 236% using a reconstituted cytochrome P4502B1:NADPH-P450 reductase system by adding to the reaction mixtures their own products. The assays were irradiated during the reactions with the excitation wavelength maximum of their products umbelliferone (lambda E = 365 n...

Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is...