A cDNA clone for cystathionine gamma-lyase was isolated from a rat cDNA library in lambda gt11 by screening with a monospecific antiserum. The identity of this clone, containing 600 bp proximal to the 3'-end of the gene, was confirmed by positive hybridization selection. Northern-blot hybridization showed the expected higher abundance of the corresponding mRNA in liver than in brain. Two furthe...

Entamoeba histolytica is an intestinal parasite causing significant morbidity and mortality worldwide. More tools are needed to understand the epidemiology and molecular pathogenesis of amebiasis. A cDNA library was constructed by using poly(A)+ RNA isolated from an axenic strain of E. histolytica, HM1:IMSS, which expresses a pathogenic isoenzyme pattern (zymodeme). Differential screening of th...

We have previously reported the sequence of a cDNA clone for a DR beta 1 chain obtained from mRNA of the DR4-Dwl4 homozygous typing cell LS40 (1). Here we report the sequence of a second clone obtained from the same library. Comparison of the sequence of this clone, LSI.1.1, with our previously published sequence and a DR beta 1 genomic sequence suggests that this clone is also a product of the...

A unique community of bacteria colonizes the dorsal integument of the polychaete annelid Alvinella pompejana, which inhabits the high-temperature environments of active deep-sea hydrothermal vents along the East Pacific Rise. The composition of this bacterial community was characterized in previous studies by using a 16S rRNA gene clone library and in situ hybridization with oligonucleotide pro...

Genus and Species. Bos indicus and Bos taurus. Locus Name. Bovine stearoyl-coenzyme A desaturase (SCD). Source and Description of Primers. Primers were designed that amplified a 135-bp region in SCD exon 6. Sequences from human (GenBank accession number Y13647), rat (AB003357), mouse (M21285), and pig (Z97186) were aligned to identify a conserved region in which to design the primers. The forwa...

The prokaryotic community composition of activated sludge from a seawater-processing wastewater treatment plant (Almeria, Spain) was investigated by using the rRNA approach, combining different molecular techniques such as denaturing gradient gel electrophoresis (DGGE), clone libraries and in situ hybridization (FISH and CARD-FISH). Most of the sequences retrieved in the DGGE and the clone libr...