the aim of this study was to compare the effect of time of parthenogenetic activation (22 hr versus 27 hr after in vitro maturation-ivm) on in vitro development of ovine oocytes using either single (ionomycin 5 ?m for 5 min or ethanol 7% for 7 min) or combined (ionomycin and ethanol with 6-dmap 2 mm for 3 hr) activation treatments. the abattoir-derived in vitro matured activated oocytes were cu...

Abstract Study question Does blastocyst-stage embryo transfer in fresh and frozen cycles improve the cumulative live birth rate (cLBR) compared with cleavage-stage IVF/ICSI treatments? Summary answer In good prognosis IVF patients (≥4 available embryos), a policy did not result significant higher cLBR to policy. What is known already A recent Cochrane systematic review meta-analysis concluded t...

The role of cell division in the expression of muscle actin and its relationship to acetylcholinesterase (AChE) development was examined in cleavage-arrested embryos of the ascidian Styela. Muscle actin expression was detected by two-dimensional gel electrophoresis of radioactively labelled proteins and by in situ hybridization with a cDNA probe, whereas AChE activity was assayed by enzyme hist...

Centrosomin is a 150 kDa centrosomal protein of Drosophila melanogaster. To study the function of Centrosomin in the centrosome, we have recovered mutations that are viable but male and female sterile (cnnmfs). We have shown that these alleles (1, 2, 3, 7, 8 and hk21) induce a maternal effect on early embryogenesis and result in the accumulation of low or undetectable levels of Centrosomin in t...

This study aimed to verify the beneficial effect of cycloheximide (CHX) treatment on the development of porcine somatic cell nuclear transfer (NT) embryos, which were constructed with enucleated oocytes and cumulus cells by using a single direct current (DC) pulse. In the first experiment, a single DC pulse applied to the induction of fusion and activation of NT embryos gave a high fusion rate....

This research shows that myoplasmic crescent material of the ascidian egg has both functional autonomy and functional specificity in establishing the differentiation pathway of muscle lineage cells. The cytoplasmic segregation pattern in eggs of Styela plicata was altered by compression of the embryos during third cleavage. This caused a meridional division instead of the normal equatorial thir...

Previous studies have demonstrated that aneuploidy in human embryos is surprisingly frequent with 50-80% of cleavage-stage human embryos carrying an abnormal chromosome number. Here we combine non-invasive time-lapse imaging with karyotypic reconstruction of all blastomeres in four-cell human embryos to address the hypothesis that blastomere behaviour may reflect ploidy during the first two cle...

Vegetal plate specification was assessed in S. purpuratus embryos after micromere deletions at the 4th, 5th and 6th cleavages, by assaying expression of the early vegetal plate marker Endo 16, using whole-mount in situ hybridization. After 4th cleavage micromere deletions, the embryos typically displayed weak Endo16 expression in relatively few cells of the lineages that normally constitute the...

the purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (icsi) and piezo-assisted sperm injection after pretreatment with calcium ionophore (cai) on the mouse embryo development. methods: in this study, the conventional icsi and piezo-icsi procedures were used. the efficacy of the methods was examined after mouse matured oocytes were fertilized with or witho...