to develop an optimal bioprocess for benzophenanthridin alkaloids production, various metabolite engineering are studied to increase yields, in which one of them is elicitation. elicitors are biotic or abiotic molecules that have effectively stimulated the production of plant secondary metabolites in cell and organ cultures. since this plant serves as the only commercial source of morphine and ...

plant cell cultures could be used as an important tool for biochemical production, ranging from natural pigments to pharmaceutical products. anthocyanin is affected by a variety of factors. light, an important plant environmental factor, influences the anthocyanin production in vegetative plant tissues. here, we have investigated the influence of the blue laser-diode (bld) and red laser-he ne (...

in biotechnology has been concentrated to review alternative routes for the production of natural compounds. plant cell culture systems are viable alternatives for the production of secondary metabolites that are of commercial importance in food and pharmaceutical industries. however, relatively very few cultures synthesize these compounds over extended periods in amounts comparable to those fo...

background and objectives: contamination of cell lines and biological products is one of the major problems of cell culture techniques. rapid detection of mycoplasma contamination in cell culture is an important part of quality control standards in related laboratories. the aim of this study was to evaluate the efficacy of pcr in detection of myroplasma as contaminants in cell cultures and othe...

In this study the programmed cell death (apoptosis) of human neutrophilic granulocytes was investigated in the presence and absence of fetal calf serum (FCS), cycloheximide and actinomycin-D. The results show that when FCS is omitted from cultures, apart from a decrease in viability, the percentage of apoptotic cells and DNA fragmentation increases. Apoptosis is accelerated in serum withdra...

The aim of this study was to investigate the prevalence of virulence and Cytolethal Distending Toxin (CDT) genes in the Campylobacter isolates from intestinal contents and gall bladders of broilers and, to evalute their cytotoxic effects on HeLa cell cultures. These genes play important roles in bacterial adherence to intestinal mucosa, flagella-mediated motility, invasive capability and the ab...

objective(s) mesenchymal stem cells (mscs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. the present study tried to isolate and characterize mscs from goat bone marrow. materials and methods fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. passaged-3 cells were then differentiat...