The objective of this experiment was to assess the utility of a commercially available enzyme-linked immunosorbent assay (ELISA) kit for diagnosis of giardiasis in archaeological human remains. The kit, a monoclonal antibody assay, is used to detect the presence of Giardia-specific antigen 65 (GSA65) in human faeces. We utilized the assay in ancient faecal material. The material included desicc...

Laboratory confirmation of the diagnosis of Lyme disease is based on the detection of an immune response to Borrelia burgdorferi. The serodiagnosis of B. burgdorferi infection is complex and may be further confounded by the immune response to the recombinant outer surface protein A (OspA) Lyme disease vaccine. To describe how the serological response to the recombinant OspA Lyme disease vaccine...

AIM The present work aimed to develop lateral flow immunochromatographic strip (ICS) test for detection of Salmonella Enteritidis (SE) specific antibodies in chicken sera. MATERIALS AND METHODS A rapid lateral flow immunochromatographic test (LFIT) has been developed, in which SE Group D antigen labeled with the gold chloride molecules laid on the conjugate pad. Staphylococcus aureus protein ...

Fasciolosis is caused by Fasciola hepatica that affects the bile ducts and liver parenchyma of ruminants, which can result in economic loss. This study aimed to carry out the validity of the commercial kit ELISA® indirect front of the simple fecal sedimentation test used as the standard. 143 samples were collected blood and feces of cattle from Jerome, south of the Espírito Santo. Serum samples...

background: helicobacter pylori (hp) is a gram negative, spiral shaped bacterium which colonizes the gastric mucosa and induces gastroduodenal complications varying from mild gastritis with no clinical complications to peptic ulcer diseases and even gastric malignancies. the rate of hp infection is 30-50% in developed countries whereas it has been rated up to 80% of the adult population in deve...

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El objetivo del estudio fue comparar las técnicas de inmunofluorescencia indirecta (IFI) y Enzimo inmunoensayo (ELISA) para el diagnóstico Neospora caninum Dubey, Carpenter, Speer, Topper Uggla, 1988 en alpacas la sierra Perú. Se evaluó sensibilidad, especificidad, valor predictivo positivo, negativo concordancia IFI usando referencia un kit comercial ELISA competitivo (cELISA - VMRD). obtuvier...

Abstract Equine infectious anemia is a worldwide distributed disease that affects the Equide family. Commercial effective vaccine not available, for reason control of depends on diagnostic tools. To improve efficiency program in Cuba, LABIOFAM Group, developed an indirect enzyme-linked immunosorbent assay (ELISA), ELISA kit, to complement system currently uses agar gel immunodiffusion (AGID) ki...

Patients with naive CHC were included in the study. All of patients genotype 1b and all them treated pegylated interferon ribavirin combined. 43 people patient group control group. Of both groups, 23 male 20 female. The mean age was 49.54±13.02, 47.98±14.1. Serum alanine aminotransferase (ALT), TNF-alpha, TGF-beta, IL-10 values studied groups. To measure TGF-beta values, 10 cc venous blood take...

BACKGROUND Brucella ovis causes an infectious disease responsible for infertility and subsequent economic losses in sheep production. The standard serological test to detect B. ovis infection in rams is the complement fixation test (CFT), which has imperfect sensitivity and specificity in addition to technical drawbacks. Other available tests include the indirect enzyme-linked immunosorbent ass...