In this study, we prepared and characterized a callus extract from Citrus junos and assessed its utility as a source of topical anti-aging ingredients. Callus extract was produced by aqueous extraction from Citrus junos grown on Murashige and Skoog medium with picloram as a growth regulator. After measuring the total phenolic and flavonoid contents, the major phenolic compound in calli was iden...

genus onobrychis is an important forage crop consisting of approximately 130 annual and perennial species. the present research focused on the cytological studies of embryogenic (e) and non-embryogenic (ne) callus as well as root meristem cells of in vitro and in vivo grown plants. on the contrary to the previous studies, a diploid viciifolia variety with 2n= 2x= 16 chromosomes was observed for...

Chrysanthemum nodal explants were cultured on 1⁄2 X MS medium containing different combinations of growth hormones. A reasonable callus formed on explants when MS contained 0.5 mg. cm each of BAP and NAA after about 3 weeks. Similar results were obtained on MS + 0.5 mg. cm BAP + 0.1 mg. cm NAA or 1.0 mg. cm BAP + 0.1 mg. cm NAA. A maroonish embryogenic callus was formed on MS containing BAP onl...

Lycopene is present in a range of fresh fruits and vegetables, especially in the leaves of Barringtonia racemosa. The traditional lycopene extraction from the plant is being employed instead of an easy propagation technique like cell culture process from the leaf explants. We intend to assess how lycopene could be extracted via tissue culture under light (illuminance: 8,200 lux under white fluo...

In the last years many studies on anthocyanins, the flavonoids responsible for the orange, red and blue colors in different plant organs, have revealed their antioxidant activity and a possible use as chemotherapeutics. The finding that tart cherries (Prunus cerasus L.) contain a great amount of anthocyanins, constituents that possess strong antioxidant and anti-inflammatory activities, have at...

During plant growth and development, the phytohormone auxin induces a wide array of changes that include cell division, cell expansion, cell differentiation, and organ initiation. It has been suggested that the actin cytoskeleton plays an active role in the elaboration of these responses by directing specific changes in cell morphology and cytoarchitecture. Here we demonstrate that the promoter...

The low yield and high market price of the pharmaceutically important alkaloids of Catharanthus roseus (L.) G. Don viz. vincristine, vinblastine and ajmalicine have created interest in improved alternative routes for their production such as using cell and tissue culture. The callus developed on Murashige and Skoog (MS) media supplemented with different concentrations of auxins and cytokinins w...

background and objectives: grape has high nutritional value and it is an economically important horticultural plant in the world. iran has seventh producer grape rank in the world. today tissue culture is one of technique which can be used in grape propagation and breeding. material and method: in order to optimization of callus induction and micropropagation of grape in vitro condition, this i...

Toddalia asiatica(Rutaceae) is a woody liana used traditionally in the treatment of malaria, sprains, cough, fever, neuralgia, epilepsy, dyspepsia and other disease conditions. The extracts of the plant have been reported to have anticancer and anti-HIV activities. These activities are mainly attributed to benzophenanthridine alkaloid i.e., nitidine. The yield of the nitidine in intact plant is...

The omission of sucrose from the basal medium stimulated callus formation in bud explants of Citrus sinensis (L.) Osbeck. Moreover, it increased the abscisic acid-induced callus proliferation reported earlier in the presence of 5% sucrose (Altman and Goren, Physiol. Plant. 32: 55, 1974). The inhibition of callus formation by the addition of sucrose was not due to the high osmotic potential of t...