The extracellular biofilm matrix includes primarily DNA and exopolysaccharides (EPS), which function to maintain aggregate structures and to protect biofilms from antibiotics and the immune response. Both polymers are anionic and have cation binding activity, however the impact of this activity on biofilms is not fully understood. Host cell contact is considered the primary signal for activatio...

in this study, chondrocyte culture was established for the first time in iran, and calcium alginate was used for longer culture of chondrocyte in vitro. the study was programmed in order to be used for future human chondrocyte transplantation. the cartilage specimen obtained from 50 patients who underwent total knee and hip operations in isfahan university of medical sciences. cartilage specime...

The main objective of the study was to design a non-condensing polymeric microspherebased gene delivery system for targeting macrophages. An external gelation technique was used to prepare plasmid DNA-encapsulated calcium alginate microspheres. The microspheres were characterized for particle size, surface charge, surface morphology, and DNA loading efficiency. Stability of encapsulated DNA was...

This paper reports a unique approach to immobilize mammalian cell populations (mouse myeloma NS0) through in situ gelation of calcium alginate triggered by an electrical signal under physiologically relevant conditions. This is the first experimental observation to evaluate the electrically triggered assembly of calcium alginate gel for entrapping and culturing mammalian cells. Subsequent cell ...

background: environmental problems of the plastic materials used in food packaging and the demand for food quality improvements lead to the development of packaging in natural materials. edible films such as calcium alginate extend the shelf life by acting as a selective barrier against moisture and oxygen. the main objective of this study was to explore a calcium alginate based edible film to ...

Floating calcium alginate beads based on gas-generation technique often suffer from poor drug encapsulation and rapid drug release in the acidic environment of the stomach. To overcome the above mentioned demerits, in the present study we have evaluated the influence of formulation factors such as incorporation of mixtures of gasgenerating agents (NaHCO3 and CaCO3), incorporation of gelatin typ...

A new emulsion gelation method was used to prepare gel beads for a highly water-soluble drug metformin hydrochloride using sodium alginate as the polymer. The gel beads containing oil was prepared by gently mixing or homogenizing oil and water phase containing sodium alginate which was then extruded into calcium chloride solution to produce gel beads. The effects of factors like type of oil and...

The main purpose of this study is developed and drug loaded nanoparticles of azelastine hydrochloride, an antiallergic drug and optimization in terms of chemical properties, drug concentration, polymer concentration, cross‐linking agent and stirring time. Nanoparticles of azelastine hydrochloride were fabricated using chitosan and sodium alginate as polymers by ionotropic pregelation method. Ca...

A study was designed to assess the performance of various swabs and transport media routinely used to collect specimens submitted for Bordetella culture and PCR. Calcium-alginate swabs inhibited the PCR. No inhibition was detected in any PCRs with dacron or rayon swabs. All swab materials performed similarly for recovery of Bordetella pertussis in culture. The Amies with charcoal transport syst...

In the present work, a gastroretentive in situ gelling liquid formulation for controlled delivery of ranitidine was formulated using sodium alginate (low, medium and high viscosity grades), calcium carbonate (source of cations) and ranitidine. Prepared formulations were evaluated for viscosity, buoyancy lag time and buoyancy duration, drug content and in vitro drug release. Formulation variable...