The present study aimed at exploring the potential of the P-glycoprotein (P-gp) transporters as a barrier to the repaglinide (REG) epithelial permeability. In-vitro intestinal absorption models, the everted gut sac, and Caco-2 cell line, were used to study the possible role of P-gp in intestinal transport of REG. In the everted gut sacs, apparent permeability coefficients showed cargo concentra...

confluent monolayers of well-differentiated enterocyte-like cells with functional properties of transporting epithelia, and retains various influx and efflux transporters expressed in the intestine, such as H -coupled oligopeptide transporter 1 (PEPT1), P-glycoprotein (P-gp), multi-drug resistance proteins (MRPs), and breast cancer resistance protein (BCRP). Caco-2 cells have been widely used a...

The concentrations of organic carbon (OC) and CaCO 3 in lake sediments are often inversely related. This relation occurs in surface sediments from different locations in the same lake, surface sediments from different lakes, and with depth in Holocene sediments. Where data on accumulation rates are available, the relation holds for organic carbon and CaCO 3 accumulation rates as well. An increa...

background: numerous molecules in papaveraceae family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few cases. objective: numerous molecules in this family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few...

Rice bran lectins, named as RBA1 and RBA2, were isolated from Oryza sativa in two chromatography steps: affinity chromatography and cation-exchange chromatography. RBA1 was found to be composed of a covalently linked heterodimer of 20- and 12-kDa subunits, and RBA2 was a noncovalently linked dimer of 12-kDa subunits. Both RBA1 and RBA2 bound to desialylated complex glycoproteins such as fetuin,...

In this research, a sensitive and reliable LC-MS/MS method was developed and applied to determine the concentration of triptolide in rat plasma, microsomes, and cell incubation media. The absolute oral bioavailability of triptolide is 63.9% at a dose of 1 mg·kg-1. In vitro, the bidirectional transport of triptolide across Caco-2 cells was studied. A markedly higher transport of triptolide acros...

A simple, reliable, and user friendly system was established to cultivate Caco-2 cell monolayer for epithelial transport studies. After an initial growth period of 1 week in a CO(2) incubator, Caco-2 cells were cultivated in an automated continuous perfusion system (Minucells and Minutissue, Germany). Medium was constantly renewed at the apical and basal side of the monolayers, which resulted i...

Structured lipids have been proposed as efficient vehicles for the supplementation of essential fatty acids (EFA) to patients with malabsorption. We investigated how a novel structured triglyceride (STG), containing purely octanoic acid in the sn-1/ sn-3 and [14C]linoleic acid in the sn-2 positions, was incorporated into different lipid classes in Caco-2 cells. We also evaluated the contributio...

Bu çalışmada N-benziletilendiamin ile 2-Hidroksi-5-metilbenzaldehit, 2-Hidroksi-5-metilasetofenon, 2-Hidroksi-5-kloroasetofenon bileşiklerinin kondenzasyon reaksiyonu sonucu üç yeni Schiff bazı sentezlendi (L1, L2 ve L3). Sentezlenen bileşiklerin yapıları, 1H NMR, 13C FT-IR elemental analiz yöntemleri karakterize edildi. Daha sonra, elde edilen sitotoksik aktiviteleri, insan kolon kanser hücre ...

L-Trp and its derivatives were used as model compounds to clarify structural factors which influence the intestinal epithelial permeation and metabolism of amino-acid derivatives. Permeability of model compounds through Caco-2 cells was used as an in vitro absorption model for human intestinal epithelial cells. The influence of compound concentration, the effects of various transporter substrat...