A cross-sectional epidemiological study was conducted to determine the seroprevalence and to identify risk factors for bovine brucellosis seropositivity in traditional and smallholder dairy cattle production systems in the Tanga region of North-eastern Tanzania. The study populations comprised 246 indigenous and 409 crossbred cattle, randomly selected from 105 smallholder dairy and 25 tradition...

Brucellosis is one of the most important reemerging zoonoses in many countries. Brucellosis is caused by Gram-negative coccobacillus belonging to genus Brucella. Human brucellosis often makes the diagnosis difficult. The symptoms and clinical signs most commonly reported are fever, fatigue, malaise, chills, sweats headaches, myalgia, arthralgia, and weight loss. Some cases have been presented w...

Ten striped dolphins, Stenella coeruleoalba, stranded along the Costa Rican Pacific coast, had meningoencephalitis and antibodies against Brucella spp. Brucella ceti was isolated from cerebrospinal fluid of 6 dolphins and 1 fetus. S. coeruleoalba constitutes a highly susceptible host and a potential reservoir for B. ceti transmission.

An enzyme immunoassay (EIA) was developed for detecting Brucella antibodies in milk of cows infected with Brucella abortus. The enzyme immunoassay using heat-killed cells of B. abortus strain 19 was of comparable sensitivity to the Brucella ring test in detecting antibodies in milk of a reference positive control cow experimentally infected with B. abortus strain 2308 and in milk of 16 naturall...

background and objective: brucellosis is a zoonotic disease of worldwide distribution and has great economic importance. despite its control in many countries, it remains endemic in iran. brucellosis was investigated in many high risk occupational groups; however, few studies on the prevalence of brucellosis among blood donors are available. to determine the seroprevalence of brucellosis antibo...

A total of 183 cattle comprising 54 bulls, 53 milking cows, 76 heifers and 44 calves in the Akwapim-South district of Ghana were tested for antibodies against Brucella abortus using the Rose Bengal plate test. The results indicated that cattle in the Akwapim-South district were infected with Brucella with a mean seroprevalence of 6. 6%. There was no difference in the seroprevalence either betwe...

background and objectives: brucella melitensis infection is still a major health problem for human and cattle in developing countries and the middle east. materials and methods: in this study, in order to screen immunogenic candidate antigens for the development of a brucella subunit vaccine, a cytoplasmic protein (dnak) and an outer membrane protein (omp31) of b. melitensis were cloned, expres...

brucellosis is a widespread zoonotic disease causing considerable economic and public health problems. despite animal vaccination, brucellosis remains endemic in some areas such as iran, especially in the western iranian province of hamadan. we sought to detect some of the most common virulence-associated genes in brucella isolated from human blood cultures to determine the prevalence of some v...

To determine the frequency of anti-Brucella canis antibodies in dogs attended in veterinary clinics from Patos, Paraíba State, Brazil, as well as to identify risk factors and to isolate and identify the agent, 193 dogs were used. Agar gel immunodiffusion test (AGID) was used to detect B. canis antibodies in sera. Isolation of B. canis was carried out in blood and bone marrow from seropositive a...

BACKGROUND Many serological tests have been used for the diagnosis of human brucellosis. A new serological method is identified as Brucella Coombs gel test based on the principle of centrifugation gel system similar to the gel system used in blood group determination. In this system, if Brucella antibodies were present in the serum, antigen and antibody would remain as a pink complex on the gel...