clostridium perfringens is an important cause of bacterial food poisoning worldwide. the disease is caused by c. perfringens enterotoxin (cpe) encoded by cpe gene. the aim of this research was to identify the different types of c. perfringens and the presence of cpe gene in isolated bacteria from broilers’ meat marketed in retail meat shops of mashhad city in northeastern of iran. after isolati...

Repetitive sequence-based PCR (rep-PCR) has been recognized as an effective method for bacterial strain typing. Recently, rep-PCR has been commercially adapted to an automated format known as the DiversiLab system to provide a reliable PCR-based typing system for clinical laboratories. We describe the adaptations made to automate rep-PCR and explore the performance and reproducibility of the sy...

Background Many bacteria and archaea possess a defense system called clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (CRISPR-Cas system) against invaders such as phages or plasmids. This system has not been demonstrated in Helicobacter pylori. The numbers of spacer in CRISPR array differ among bacterial strains and can be used as a genetic marker for bacte...

Protein fingerprinting is a widely used technique in epidemiological studies for typing bacterial strains. This study reports the development of a computer based gel analysis system. The system has the capability to analyse SDS-PAGE whole-cell protein profiles using digital image processing techniques. The software incorporates spatial and frequency domain operators for image enhancement, suppo...

The aim of this study was to identify and compare the plasmid replicons clinical uropathogenic Escherichia coli (UPEC) isolates, involving extended spectrum β-lactamase (ESBL)-positive ESBL-negative, E. ST131 non-ST131 various subclones. Plasmid replicon typing on 24 UPEC isolates carried out using polymerase chain reaction-based typing. A statistical analysis performed assess associations betw...

Whole genome sequencing has become one of the routine methods in molecular epidemiological practice. In this study, we present BacWGSTdb (http://bacdb.org/BacWGSTdb), a bacterial whole genome sequence typing database which is designed for clinicians, clinical microbiologists and hospital epidemiologists. This database borrows the population structure from the current multi-locus sequence typing...

Stenotrophomonas maltophilia is an opportunistic nosocomial pathogen and is being isolated worldwide with increasing frequency. S. maltophilia isolates are often highly resistant to most of the currently used antimicrobial agents, including carbapenems, aminoglycosides, and fluoroquinolones. A variety of antimicrobial resistance mechanisms, including efflux pumps and integrons, have been report...

Pyocine production typing and restriction fragment length polymor¬ phism (RFLP) of plasmid DNA with BamHl (BamHl RFLP) were com¬ pared for intraspecies discrimination of 1 00 Pseudomonas aerugi¬ nosa isolâtes. Typeability of pyocine production method was 76% while that of BamHl RFLP was 100%. BamHl RFLP was highly discriminative so as to distinguish unrelated isolâtes of close lineage. However,...

This paper presents several common molecular typing methods, Pulsed-Field Gel Electrophoresis (PFGE), Restriction Fragment Length Polymorphisma (RFLP), Random Amplified Polymorphic DNA (RAPD), Automatic Ribotyping, rep-PCR, Multi-Locus Sequence Typing (MLST), in which the application and progress of molecular typing of pathgens for these methods are described. These methods played an important ...

The PCR-based methodology applied to multiple-locus variable numbers of tandem repeat (VNTR) analysis was recently shown to be a useful technique for the molecular typing of clinical isolates of several bacterial species. We have adopted this method for the molecular typing of methicillin-resistant Staphylococcus aureus. Five staphylococcal VNTR loci (sdr, clfA, clfB, ssp, and spa) were subject...