AIM To construct the expression vector of B3 (scdsFv)-SEA (D227A) and to identify its binding and cytotoxic ability to B3 antigen positive carcinoma cell lines. METHODS This fusion protein was produced by a bacterial expression system in this study. It was expressed mainly in the inclusion body. The gene product was solubilized by guanidine hydrochloride, refolded by conventional dilution met...

Innate emotion response to environmental stimuli is a fundamental brain function that is controlled by specific neural circuits. Dysfunction of early emotional circuits may lead to neurodevelopmental disorders such as autism and schizophrenia. However, how the functional circuits are formed to prime initial emotional behaviours remain elusive. We reveal here using gene-targeted mutations an ess...

It is well known by many clinicians in the field of complementary and alternative medicine that vitamin B3 has numerous therapeutic indications. The many review articles, case reports, and books authored by Hoffer fully elucidate the known and theoretical benefits of vitamin B3 administration for the treatment of AIDS, alcoholism, arthritis, cancer, cardiovascular disease, childhood learning an...

Site-specific recombinases have been used for two decades to manipulate the structure of animal genomes in highly predictable ways and have become major research tools. However, the small number of recombinases demonstrated to have distinct specificities, low toxicity, and sufficient activity to drive reactions to completion in animals has been a limitation. In this report we show that four rec...

A set S of integers is a B3-set if all the sums of the form a1 + a2 + a3, with a1, a2 and a3 ∈ S and a1 ≤ a2 ≤ a3, are distinct. We obtain asymptotic bounds for the number of B3-sets of a given cardinality contained in the interval [n] = {1, . . . , n}. We use these results to estimate the maximum size of a B3-set contained in a typical (random) subset of [n] of a given cardinality. These resul...

1 This study examines b1-, b2and b3-adrenoceptor (AR)-mediated responses, mRNA levels and radioligand binding in ileum from b3-AR knock-out (7/7) (KO) and wild type (+/+) (FVB) mice. 2 In KO and FVB mice, SR59230A (100 nM) (b3-AR antagonist) antagonized responses to (7)isoprenaline in both KO and FVB mice. (7)-Isoprenaline mediated relaxation of ileum was antagonized weakly by ICI118551 (100 nM...

1 Radioligand binding and patch-clamp techniques were used to study the actions of g-aminobutyric acid (GABA) and the general anaesthetics propofol (2,6-diisopropylphenol), pentobarbitone and 5a-pregnan3a-ol-20-one on rat a1 and b3 GABAA receptor subunits, expressed either alone or in combination. 2 Membranes from HEK293 cells after transfection with a1 cDNA did not bind signi®cant levels of [S...

tb-triple = {planta part-of floresta} (plant part-of forest) A1: relação, quadro, planta, mapa B1: bosque, floresta, mata, brenha, selva A2: vegetal, planta A3: traçado, desenho, projeto, planta, plano plausible sb-triples = {A2, B1} tb-triple = {passageiro purpose-of carruagem} (passenger purpose-of carriage) A1: passageiro, viajante B1: carriagem, carruagem, carraria A2: passageiro, viador B2...

There is no one to one 1-Lipschitz function from S2 to the interval [0,1] because of 1.2. Is there finite to one 1-Lipschitz function from S2 to the interval [0,1]? Is there countable to one 1-Lipschitz fuction from S2 to the interval [0,1]? The answers are no for both questions becase of 5.3. In this paper, I define a family of new metrics on the Euclidean 2-sphere of radius r in which the dis...

BACKGROUND/PURPOSE In recent years, coxsackievirus B3 (CV-B3) has been determined as a dominant enterovirus serotype that may cause severe complications in patients. Since 2008 in Taiwan, some enterovirus isolates have been regarded as untypeable [by employing commercial immunofluorescence assay (IFA) kits]. In 2012, the number of isolates increased. Genetic sequence analysis further confirmed ...