AIM To evaluate the efficacy of recombinant outer membrane proteinC (rOmpC) based enzyme-linked immunoassay (ELISA) for the diagnosis of salmonellosis in poultry. MATERIALS AND METHODS Three antigens were prepared, and the indirect ELISA was standardized using the antigens and the antiserum raised in chicken against Omp and rOmpC. Sera were collected from a total of 255 apparently healthy fie...

Actinobacillus pleuropneumoniae is the etiological agent of Porcine pleuropneumonia, which causes severe losses in pig farming. To establish an serological method to detect A. pleuropneumoniae infection, bioinformatics method was utilized to analyzed the sequence of apxIVA gene of A. pleuropneumoniae serotype 5 completed by our lab, a 1152 bp fragment of the N'-terminal of apxIVA gene was ampli...

Although winter dysentery (WD) has been suspected to occur frequently in Korea, to date the exact epidemiology of WD has remained unknown. Therefore, we investigated the causative agents of WD by using electron microscopy, ELISA, RT-PCR, and nested PCR from 97 fecal samples of 32 WD-affected herds collected from 8 provinces during 2002-2004. The bovine coronavirus (BoCV) was consistently detect...

The purpose of the current study was to identify potential ligands and develop a novel diagnostic test to highly pathogenic avian influenza A virus (HPAI), subtype H5N1 viruses using phage display technology. The H5N1 viruses were used as an immobilized target in a biopanning process using a 12-mer phage display random peptide library. After five rounds of panning, three phages expressing pepti...

Infection with Neisseria meningitidis group B has been difficult to detect, partly because this bacterial group's polysaccharide is a weak immunogen. This article describes work carried out to test a new procedure (MB-Dot-ELISA) employing a high-titered horse antiserum for detection of N. meningitidis group B antigens. The study assayed cerebrospinal fluid samples from 585 subjects, 574 with su...

Comparative tests using inmunosorbent electron microscopy plus decoration and ELISA with antibodies against Garlic virus A (GarV-A) and Garlic virus C (GarV-C) coat proteins showed that they specifically recognize the respective viruses. On this basis, the GarV-A antiserum was used in DAS-ELISA tests to study the distribution of GarV-A in different regions of Argentina. A total of 2655 samples,...

This report describes an approach for the study of the biology of methylarginine proteins based on the generation of immunological reagents capable of recognizing the methylarginine status of cellular proteins. Two forms of an immunizing peptide were prepared based upon an amino acid sequence motif found most prevalently among verified dimethylarginine-containing proteins. One form of the pepti...

Methods for the evaluation of root rot disease in sugarbeet caused by pathogenic fungi historically have relied on visual assessment. In an initial attempt to develop complementary means to detect and quantitate root rot disease caused by Aphanornyces cochlioides, antiserum was produced in rabbits that had been immunized with a cell-wall preparation of this organism. Specificity tests using enz...

An enzyme-linked immunosorbent assay using a homologous combination of antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin (T-3-0-CMO-BSA ) and penicillinase-labelled T-3-0-CMO was developed. This assay was utilized to measure testosterone in serum samples of male and female subjects. The sensitivity of the assay is 50pg/well and the antibody developed crossrea...

AIM To develop a highly sensitive and specific enzyme linked immunosorbent assay (ELISA) system for analysis of p53 protein in cancer lysates. METHODS The anti-p53 monoclonal antibodies DO7, 1801, BP53.12, and 421, and anti-p53 polyclonal antiserum CM1 were assessed by immunohistochemistry and western blot analysis to identify those most suitable for determining p53 status of cancer cells. An...