Plant propagation originated  from the mature tissues is the most desirable method in producing clonal plants, however microbial contamination usually is the main concern during regeneration processes. Moreover, commercial production of plants using clonal propagation is considered as high throughput method due to the   removal of seasonal barriers, lower cost of production with higher yield wh...

As sessile organisms, plants must respond to the environment by adjusting their growth and development. Most of the plant body is formed post-embryonically by continuous activity of apical and lateral meristems. The development of lateral adventitious roots is a complex process, and therefore the development of methods that can visualize, non-invasively, the plant microstructure and organ initi...

De novo root organogenesis is the process in which adventitious roots regenerate from detached or wounded plant tissues or organs. In tissue culture, appropriate types and concentrations of plant hormones in the medium are critical for inducing adventitious roots. However, in natural conditions, regeneration from detached organs is likely to rely on endogenous hormones. To investigate the actio...

BACKGROUND Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioinformatics tools with massively parallel sequencing technology. Typically, this involves mapping or BLASTN searching individual reads against viral nucleotide databases. Althoug...

Browning and contamination are regarded as the main constraints in plant tissue culture of Musa spp. that can hinder success propagation vitro. is caused by enzymatic reactions due to explant injury, while microbial phyllospheric, rhizospheric, endophytic microorganisms reside on, in, inside plants. When not properly addressed, they cause decreased regenerative ability, callus growth, inhibited...

The appropriateness of existing methodologies to assess the potential risks associated with engineered and adventitious products of nanotechnologies

A seed high-lysine trait is negatively associated with the TCA cycle and slows down Arabidopsis seed germination. Malvar TM. 2008. Modifying lysine biosynthesis and catabolism in corn with a single bifunctional expression/silencing transgene cassette. 1995. The lysine-dependent stimulation of lysine catabolism in tobacco seed requires calcium and protein-phosphorylation. Expression of de novo h...

Root formation and root architecture of olive microshoots, inoculated or not with Pseudomonas fluorescent P19 or P21, were evaluated by measuring of length and the numbers of adventitious and lateral roots. Three-four nodal in vitro shoots were treated with different population densities (0, 105, 108 CFUml-1) of rhizobacteria. The density of 108 CFUmgl-1 induced almost two times increase in num...

A hydroponic experiment was carried out to compare root anatomy and hydraulic conductivity of four barley cultivars including Valfajr, Karoon, Afzal and Zarjo under salt stress conditions. The results showed that under salt stress, the minimum diameter of vessels was observed in the peripheral metaxylem of seminal roots of Valfajr cultivar and in adventitious roots; Karoon with 19±3 µm had maxi...